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Effect of POLDIP2 knockout in ARPE-19 cells

ABSTRACT: Effect of POLDIP2 knockout in ARPE-19 cells

PROVIDER: PRJNA853957 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19895500.fastq.gz	Fastqsanger.gz
	SRR19895501.fastq.gz	Fastqsanger.gz
	SRR19895502.fastq.gz	Fastqsanger.gz
	SRR19895503.fastq.gz	Fastqsanger.gz
	SRR19895504.fastq.gz	Fastqsanger.gz

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Effect of POLDIP2 knockout in ARPE-19 cells

Project description:To study the function of AMD-associated gene POLDIP2 in retinal pigment epithelial (RPE) cells, we used CRISPR/Cas to knockout POLDIP2 in the human RPE cell line ARPE19. We then performed RNA-seq to profile the transcriptome of wildtype ARPE19 and POLDIP2 knockout.

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Characterization of novel whole body Poldip2 knockout mice generated by [Precisely "flox"ing Poldip2 gene] and crossing with Cre deleter mice

Project description:Poldip2 is a multifunctional protein whose roles are only partially understood. The previous Poldip2 knockout mouse generated by the gene trap model suffers from two limitations: perinatal lethality in homozygotes and constitutive Poldip2 inactivation. To overcome these limitations, we developed a new conditional floxed Poldip2 mouse. Here, we correctly targeted floxed Poldip2 mice to produce a new constitutive knockout line by crossing with a Cre deleter. In contrast to the gene trap model, many homozygous knockout mice were viable, in spite of having no Poldip2 expression. To characterize the effect of Poldip2 ablation in the vasculature, we performed a RNA-seq study using the carotid arteries of these mice.

2021-12-20 | GSE165274 | GEO

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Project description:Poldip2 is a multifunctional protein whose roles are only partially understood. The previous Poldip2 knockout mouse generated by the gene trap model suffers from two limitations: perinatal lethality in homozygotes and constitutive Poldip2 inactivation. To overcome these limitations, we developed a new conditional floxed Poldip2 mouse. Here, we correctly targeted floxed Poldip2 mice to produce an endothelial-specific by crossing it with CDH5 ERT2 Cre mouse. Additionally, we also produced smooth-muscle specific Poldip2 knockout mice by crossing the flox'ed Poldip2 mice with SM22A-Cre mice. The Poldip2_EC-/- and Poldip2_SMC-/-mice were viable and showed remarkably reduced Poldip2 expression. To characterize the effect of Poldip2 ablation in the ECs and in the SMCs, we performed a RNA-seq study using the carotid arteries of these mice.

2024-01-24 | GSE165939 | GEO

Expression Data from DMSO or SRPIN803 treated ARPE-19 cells

Project description:We used microarrays to evaluate the effect of SRPIN803 on gene expression in ARPE-19 cells. ARPE-19 cells were treated with SRPIN803 (10 uM) or the negative control (0.1% DMSO) for 4 hours for Total RNA isolation and hybridization on Microarray.

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Expression Data from DMSO or SRPIN803 treated ARPE-19 cells

Project description:We used microarrays to evaluate the effect of SRPIN803 on gene expression in ARPE-19 cells.

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Project description:This study compared the gene expression change of ARPE-19 cells before and after adriamycin treatment

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Gene expression signitures in ARPE-19 cells treated with or without TGF-β1

Project description:To identify the dysregulated lncRNA and mRNA expression in ARPE-19 cells underwent EMT, we established a TGF-β1 induced EMT model of ARPE-19 cells. ARPE-19 cells were treated with or without 10 ng/ml TGF-β1 for 48 h. Total RNA are extracted and subjected to microarray assay (Arraystar Human LncRNA Microarray V3.0)

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