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Ebola virus

ABSTRACT: Linked variations in the Ebola virus polymerase are associated with organ specific phenotypes

PROVIDER: PRJNA856238 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22390800_1.fastq.gz	Fastqsanger.gz
	SRR22390800_2.fastq.gz	Fastqsanger.gz
	SRR22390900_1.fastq.gz	Fastqsanger.gz
	SRR22390900_2.fastq.gz	Fastqsanger.gz
	SRR22390801_1.fastq.gz	Fastqsanger.gz

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Project description:The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with Zaire Ebola virus and mutants: Zaire Ebola virus: This wild-type Ebola virus - strain Mayinga - was isolated from a fatal human case in Zaire (now known as the Democratic Republic of Congo) in 1976 Zaire Ebola virus, VP35 R312A possesses a R312A mutation in the VP35 protein. Zaire Ebola virus, delta sGP. Lacks the ability to produce non-structural protein, the secreted glycoprotein (sGP). Zaire Ebola virus, delta mucin. Lacks the mucin-like domain (MLD), which contains both N-linked and O-linked glycosylation sites, for the glycoproteins. Overview of Experiment: Cells: Immortalized Human Hepatocytes (IHH); seed 60,000 cells per well in a 24-well plate. Infected with a multiplicity of infection (MOI) of 0.5. After infection, 3x wash with PBS and replace with 5% FCS DMEM without NaPyr or NEAA. Time matched mocks done in triplicate from same cell stock as rest of samples. Time Points = 0, 6, 12, 24, 48, and 72 hrs post infection in triplicate.

2015-06-18 | E-GEOD-69943 | biostudies-arrayexpress

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Immune parameters and outcome during Ebola virus disease

Project description:Immune patterns in Ebola patients were characterized depending on the outcome of the illness. Non-healthy controls were compared to Ebola patients to define the specificity of the immune response against Ebola virus infection.

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Project description:F2 female mice were generated from a cross of two collaborative cross (CC) strains, CC011 x CC074. These mice were infected with Ebola virus and liver samples were prepared at day three after infection. RNA from the livers were prepared and subjected to RNAseq analysis. This data set can be combined with data from GSE165142, in which female mice from the same cross were infected with Ebola virus, and liver samples were prepared at day six after infection. Results: Variations in gene expression levels that may be perdictive for severity

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Human U937 cell transcriptome response to Zaire Ebola virus wild-type in the delta-VP30 background and delta-mucin virus

Project description:The purpose is to obtain samples for mRNA analysis in human U937 cells infected with Zaire Ebola virus wild-type in the deltaVP30 background and delta-mucin virus. Human U937 cells (monocyte-like) expressing the Ebola VP30 protein were infected with Zaire Ebola virus wild-type (wild) in the delta-VP30 background and delta-mucin virus (encodes a GP lacking the mucin domain) (mucin). Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 8, 18, and 30 h post-infection.

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