Project description:Listeria monocytogenes is the ubiquitous food-borne pathogen which causes listeriosis, a disease with a high mortality rate, mostly transmitted through contaminated ready-to-eat foods (EFSA, 2018). To better understand the systemic response of such microorganism exposed at three environmental factors (T, pH and NaCl), the proteome of a L. monocytogenes strain, which was isolated from a meat product (Coppa di testa) linked to a listeriosis outbreak occurred in Marche region (Italy) in 2016, was investigated in order to identify differences in its protein patterns.
2022-08-12 | PXD033519 | Pride
Project description:Opportunistic pathogen that causes important food-borne disease
| PRJNA798519 | ENA
Project description:Opportunistic pathogen that causes important food-borne disease
Project description:Listeria monocytogenes is a food-borne pathogen and the causative agent of listeriosis, an infection which typically arises through the consumption of contaminated foodstuffs. L. monocytogenes is a psychotrophic and facultatively anaerobic; properties which permit growth under refrigeration conditions and within modified atmosphere packaging. Through transcriptional changes L. monocytogenes is able to mount adaptive responses against stressors. Such responses typically cross protect against subsequent stresses.
Project description:Listeria monocytogenes is a food-borne pathogen which causes listeriosis. It is an intracellular parasite invading the epithelial cells where it escapes from the vacuole into the host cytoplasm to replicate, using actin-based motility to move within and between cells. The intracellular life cycle is well documented whereas the time spent in the lumen of the intestine is poorly understood. The aim of this study was to investigate the mechanism by which L. monocytogenes adapts to the environment of the small intestine prior to invasion. Specifically, to determine if the PrfA regulon, that encodes the virulence factors of L. monocytogenes, is switched on by signals within the intestinal lumen. L. monocytogenes were grown under aerobic or microaerobic conditions with glucose or glycerol as carbon source.
Project description:<i>Campylobacter jejuni</i>, non-typhoidal <i>Salmonella</i> spp., <i>Listeria monocytogenes</i> and enteropathogenic/enterohemorrhagic <i>Escherichia coli</i> (EPEC/EHEC) are leading causes of food-borne illness worldwide. <i>Citrobacter rodentium</i> has been used to model EPEC and EHEC infection in mice. The gut microbiome is well-known to affect gut colonization and host responses to many food-borne pathogens. Recent progress has established gnotobiotic mice as valuable models to study how microbiota affect the enteric infections by <i>S</i>. Typhimurium, <i>C. rodentium</i> and <i>L. monocytogenes</i>. However, for <i>C. jejuni</i>, we are still lacking a suitable gnotobiotic mouse model. Moreover, the limited comparability of data across laboratories is often negatively affected by variations between different research facilities or murine microbiotas. In this study, we applied the standardized gnotobiotic OligoMM<sup>12</sup> microbiota mouse model and compared the infections in the same facility. We provide evidence of robust colonization and significant pathological changes in OligoMM<sup>12</sup> mice following infection with these pathogens. Moreover, we offer insights into pathogen-specific host responses and metabolite signatures, highlighting the advantages of a standardized mouse model for direct comparisons of factors influencing the pathogenesis of major food-borne pathogens. Notably, we reveal for the first time that <i>C. jejuni</i> stably colonizes OligoMM<sup>12</sup> mice, triggering inflammation. Additionally, our comparative approach successfully identifies pathogen-specific responses, including the detection of genes uniquely associated with <i>C. jejuni</i> infection in humans. These findings underscore the potential of the OligoMM<sup>12</sup> model as a versatile tool for advancing our understanding of food-borne pathogen interactions.
Project description:L. monocytogenes is a food-borne pathogen which causes listeriosis. The aim of this study was to investigate the global gene expression changes in L. monocytogenes in response to spent and fresh media, in the wild-type and non-aggregating actA-DC strain.
Project description:This study explored the mode of action of glabridin, a prenylated isoflavan with antimicrobial activity, against L. monocytogenes, an important food-borne pathogen.
