Project description:Schwann cells undergo reprogramming after nerve injury, switching to immature repair phenotype. The goal was to test what genetic perturbations are triggered in Schwann cells with the stimulus from melanoma cell secreted factors. We used microarrays to detail the changes in gene expression of Schwann cells treated with human melanoma conditioned medium.
Project description:To understand the mechanism of action of ILB we have used gene expression analysis to document the changes induced by the drug in Schwann like cells. Human Schwann-like cells (ATCC-CRL-2884) were treated with ILB (0.01 mg/ml) for 48 hours. Control samples were cultured parallel with no drug added. The expression data was used to identify the ILB-regulated genes.
Project description:Analysis of the effect of NF1 second hit mutation to the reading of the whole human genome by comparing the gene expression profiles of neurofibroma derived Schwann cell cultures representing two different NF1 genotypes (NF1+/- and NF1-/-).
Project description:In this dataset, we include the expression data obtained from rat Schwann cells overepressing GFP (control) or Sox2/GFP to identfiy Sox2 tragets in Schwann cells. An in vitro microarray analysis of GFP (control) and Sox2/GFP overexpressing Schwann cells