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The effect of macrophage depletion on mammary basal cells

ABSTRACT: The effect of macrophage depletion on mammary basal cells

PROVIDER: PRJNA864852 | ENA |

REPOSITORIES: ENA

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The effect of macrophage depletion on mammary basal cells

Project description:To investigate the effect of macrophages on mammary basal cells, we established a macrophage depletion mouse model by clodronate liposome (CL) intraperitoneal injection, and the mammary basal cells were collected by FACS sorting. We then performed RNA-seq analysis on clodronate liposome (CL) treated mammary basal cells compared to control.

2023-04-20 | GSE210268 | GEO

Effect of laminin adhesion on basal human mammary epithelial cells

Project description:Effect of laminin adhesion on basal human mammary epithelial cells

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Effect of laminin adhesion on basal human mammary epithelial cells

Project description:Mammary epithelium is composed by luminal and basal epithelial cells, which are adhere to the basement membrane (BM). To dissect how basal cell functions are regulated by BM laminin adhesion, we performed RNA sequencing of basal human mammary epithelial cells adhered on laminin-111, -421 or -521 coated cell culture plates for 48 hours.

2022-11-07 | GSE197950 | GEO

Inactivation of LATS1/2 drives luminal-basal plasticity to initiate basal-like mammary carcinomas

Project description:Basal breast cancers, an aggressive breast cancer subtype that has poor treatment options, are thought to arise from luminal mammary epithelial cells that undergo basal-like plasticity through poorly understood mechanisms. Using genetic mouse models and ex vivo primary organoid cultures, we show that conditional co-deletion of the LATS1 and LATS2 kinases, key effectors of Hippo pathway signaling, in mature mammary luminal epithelial cells promotes the development of basal-like carcinomas that metastasize over time. Genetic co-deletion experiments revealed that phenotypes resulting from the loss of LATS1/2 activity are dependent on the transcriptional regulators YAP/TAZ. Notably, transcriptional analyses of LATS1/2-deleted mammary epithelial cells revealed a gene expression program that associates with human basal breast cancers. Our study demonstrates in vivo roles for the LATS1/2 kinases in mammary epithelial homeostasis and luminal-basal fate control and implicates signaling networks induced upon the loss of LATS1/2 activity in the development of basal breast cancers.

2022-09-26 | GSE196555 | GEO

Macrophage depletion alleviates pain-related behaviors in osteoarthritic mice

Project description:The objectives of this study were to determine the effect of macrophage depletion on pain-like behaviors, joint damage, and DRG molecular changes in both male and female mice with osteoarthritis.

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Microarray of 3D cultured basal cells co-cultured with M2-like macrophages

Project description:To facilitate the research on the interaction between mammary basal cells and M2-like macrophages, we established a co-culture system of mammary basal cells and M2-like macrophages in vitro, and performed microarray of M2-like macrophage co-cultured basal cells comparing with control, for investigating the changes of downstream signals after co-culturing with M2-like macrophages in the basal cells.

2023-04-20 | GSE210379 | GEO

Effect of depletion of NLRC3 on glycolytic genes of macrophage with LPS treatment

Project description:Effect of depletion of NLRC3 on glycolytic genes of macrophage with LPS treatment

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Effect of NCOA4-depletion on the transcriptome profile of J774 mouse macrophage cells

Project description:Effect of NCOA4-depletion on the transcriptome profile of J774 mouse macrophage cells

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Distinct lactation-associated macrophages exist in murine mammary tissue and human milk

Project description:Macrophages are involved in immune defense, organogenesis and tissue homeostasis. They also contribute to the different phases of mammary gland remodeling during development, pregnancy and involution post-lactation. Yet, less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multi-parameter flow cytometry and single-cell RNA sequencing we reveal this population as distinct from the two resident macrophage subsets present pregestationally. These lactation-induced macrophages (LiMacs) are predominantly monocyte-derived and expand by proliferation in situ concomitant with nursing. LiMacs develop independently of IL-34 but require CSF-1 signaling and are partly microbiota-dependent. Locally, they reside adjacent to the basal cells of the alveoli and extravasate into the milk. Moreover, we also found several macrophage subsets in human milk, resembling LiMacs. Collectively, these findings reveal the emergence of unique macrophages in the mammary gland and milk during lactation.

2023-06-23 | PXD041711 | Pride

Transcription Factor RFX3 Stabilizes Mammary Basal Cell Identity

Project description:Transcription Factor RFX3 Stabilizes Mammary Basal Cell Identity

| PRJNA790932 | ENA