Project description:we characterized the microbial communities and proteomes of POC collected from the twilight zone at three contrasting sites in the northwest Pacific Ocean using a metaproteomic approach.Particle-attached bacteria, Alteromonadales, Rhodobacterales and Enterobacteriales, were the major remineralizers of POC in the twilight zone.
2021-03-24 | PXD014630 | Pride
Project description:18s Sequencing in Northwest Pacific
Project description:C1q domain-containing (C1qDC) proteins are a group of biopolymers involved in immune response as pattern recognition receptors (PRRs) in a lectin manner identifying and binding carbohydrate components of pathogen-associated molecular patterns (PAMPs). In the current study, we present a new protein MkC1qDC-1 purified from the hemolymph plasma of Modiolus kurilensis bivalve mollusk widespread in the Northwest Pacific. The isolation procedure included ammonium sulfate precipitation followed by affinity chromatography on pectin-Sepharose. The full-length MkC1qDC-1 sequence was assembled based on mass-spectrometry data supplemented with N-terminal Edman sequencing, and included 156 amino acid residues displaying high homology to C1qDC proteins of bivalves. 2-D electrophoresis of purified MkC1qDC-1 showed a single polypeptide with 19 kDa mass and pI 5.2 in accordance with predicted values. The protein was functionally active after 1 h incubation in temperatures up to 50°C and stable over a wide pH range. MkC1qDC-1 demonstrated antibacterial properties against gram-negative and gram-positive strains.
Project description:We genotyped 45 new samples from 4 populations of Northwest India and combined it with previously published data to characterize the population structure of modern Northwest Indian populations in the context of their geographic neighbors across South Asia and West Eurasia.
Project description:Interventions: Case series:bevacizumab / PD-1 monoclonal antibody treatment
Primary outcome(s): 16S sequencing
Study Design: Single arm
