Project description:To investigate the differential analysis of gene expression of mutations at different sites of H2B, we constructed six strains with point mutations at different positions of H2B, and then, we performed the gene expression analysis from the RNA-Seq data.
Project description:Histone H2B was mutated to give H2B^3-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Experiment Overall Design: mutant compared to wild-type, 3 replicates
Project description:Histone H2B was mutated to give H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B HBR domain. This SuperSeries is composed of the SubSeries listed below.
Project description:Histone H2B was mutated to give H2B^30-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B HBR domain. Keywords: repeat
Project description:Histone H2B was mutated to give H2B^3-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Keywords: repeat
Project description:Histone H2B was mutated to give H2B^3-32. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Keywords: repeat
Project description:Histone H2B was mutated to give H2B K6,11,16,17,21,22G. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal acetylated lysine residues. Keywords: repeat
Project description:Histone H2B was mutated to give H2B K6,11,16,17,21,22G. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal acetylated lysine residues. Experiment Overall Design: mutant compared to wild-type, 3 replicates
Project description:Histone H2B was mutated to give H2B^3-32. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Experiment Overall Design: mutant compared to wild-type, 3 replicates