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Bos taurus breed:Canadian Holstein

ABSTRACT: Bos taurus breed:Canadian Holstein Raw RNA-Seq reads from cows with subclinical S. aureus mastitis

PROVIDER: PRJNA878880 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24593469_1.fastq.gz	Fastqsanger.gz
	SRR24593469_2.fastq.gz	Fastqsanger.gz
	SRR24593470_1.fastq.gz	Fastqsanger.gz
	SRR24593470_2.fastq.gz	Fastqsanger.gz
	SRR24593471_1.fastq.gz	Fastqsanger.gz

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Streptococcus agalactiae and Prototheca spp. infections induce changes in milk peptidome in Holstein cattle

Project description:Early detection of bovine subclinical mastitis may improve treatment strategies and reduce the use of antibiotics. Herein, individual milk samples from Holstein cows affected by subclinical mastitis induced by S. agalactiae and Prototheca spp. were analyzed by untargeted and targeted mass spectrometry approaches to assess changes in their peptidome profiles and identify new potential biomarkers of the pathological condition. Results showed a higher amount of peptides in milk positive at the bacteriological examination when compared with the negative control. However, the different pathogens seemed not to trigger specific effects on milk peptidome. The peptides that best distinguish positive from negative samples are mainly derived from the most abundant milk proteins, especially from β- and αs1-casein, but also include the antimicrobial peptide casecidin 17. These results provide new insights into the physiopathology of mastitis. Upon further validation, the panel of potential discriminant peptides could help to the development of new diagnostic and therapeutic tools.

2024-01-26 | PXD043426 | Pride

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