Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, A375]

ABSTRACT: A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, A375]

PROVIDER: PRJNA880541 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21563131_1.fastq.gz	Fastqsanger.gz
	SRR21563131_2.fastq.gz	Fastqsanger.gz
	SRR21563132_1.fastq.gz	Fastqsanger.gz
	SRR21563132_2.fastq.gz	Fastqsanger.gz
	SRR21563133_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, A375]

Project description:Developmental signaling pathways associated with growth factors such as TGFb are commonly dysregulated in melanoma. Here we identified a human TGFb enhancer that was specifically activated in melanoma cells treated with TGFB1 ligand. We generated stable transgenic zebrafish with this enhancer driving green fluorescent protein (TIE:EGFP). EGFP was not expressed in normal melanocytes or early melanomas but was expressed in spatially distinct regions of mature melanomas. Single cell RNA-sequencing revealed that TIE:EGFP+ melanoma cells down-regulated interferon response, while up-regulating a novel set of chronic TGFb target genes. AP-1 factor binding is required for activation of this chronic TGFb reporter. Overexpression of the chromatin remodeler, SATB2, which is associated with tumor spreading shows activation of TGFb signaling in melanoma precursor zones and early melanomas. Confocal imaging and flow cytometric analysis showed that macrophages are recruited to EGFP positive regions and preferentially phagocytose TIE:EGFP+ cells. This work identifies a TGFb induced immune response and demonstrates the need for the development of chronic TGFb biomarkers to predict patient response to TGFb inhibitors.

2024-06-13 | GSE213327 | GEO

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [ChIP-seq]

2024-06-13 | GSE213326 | GEO

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [SORT-seq]

2024-06-13 | GSE213359 | GEO

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, ZMEL]

2024-06-13 | GSE213328 | GEO

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma

Project description:A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma

| PRJNA880593 | ENA

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [ChIP-seq]

Project description:A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [ChIP-seq]

| PRJNA880538 | ENA

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [SORT-seq]

Project description:A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [SORT-seq]

| PRJNA880596 | ENA

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, ZMEL]

Project description:A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, ZMEL]

| PRJNA880540 | ENA

Expression data from A375 melanoma parental and persister cells

Project description:Identify transcriptionnally and translationally regulated mRNA in melanoma parental and persister cells In this dataset, we include expression data of A375 melanoma drug-naïve parental cells and A375 melanoma persister cells that survived from BRAF and MEK inhibition. The expression data are studied in both total RNA and polysome-bounded RNA.

2019-09-20 | GSE137724 | GEO

A375 melanoma cells treated with leflunomide 25uM

Project description:Human A375 melanoma cells were treated with leflunomide 25uM for 3 days, then RNA harvested 6 arrays

2011-03-30 | E-GEOD-24526 | biostudies-arrayexpress