Project description:We investigate how DNA methylation absence impact transcriptionnal regulation during epiblast differentiation. More specifically, how synchronous is the naïve to primed transitioning in TKO cells compared to WT, and if there is a "germline-competent" TKO subpopulation of EpiLCs. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation WT and TKO at D0, D1, D2 and D4. We next performed single-cell SMARTseq2 RNA sequencing.
Project description:We investigate the impact of DNA methylation on epiblast differentiation. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed bulk RNAseq at D0, D2, D4, D6 and D8 of differentiation to study transcriptomic differences.
Project description:We investigate the impact of DNA methylation on epiblast differentiation. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed bulk RNAseq at D0, D2, D4, D6 and D8 of differentiation to study transcriptomic differences.
Project description:We investigate the impact of DNA methylation on neural somatic differentiation. To this end, we used in vitro neural progenitor cell (NPC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed bulk RNAseq at D0, D2, D4, D6 and D8 of differentiation to study transcriptomic differences.
Project description:We investigate the impact of DNA methylation on chromatin epigenetic marks during epiblast differentiation. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed CUT&RUN against H3K27me3 and H3K27ac at D0, D2 and D4.