Project description:We investigate the impact of DNA methylation on epiblast differentiation. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed bulk RNAseq at D0, D2, D4, D6 and D8 of differentiation to study transcriptomic differences.
Project description:We investigate the impact of DNA methylation on epiblast differentiation. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed bulk RNAseq at D0, D2, D4, D6 and D8 of differentiation to study transcriptomic differences.
Project description:We investigate the impact of DNA methylation on neural somatic differentiation. To this end, we used in vitro neural progenitor cell (NPC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed bulk RNAseq at D0, D2, D4, D6 and D8 of differentiation to study transcriptomic differences.
Project description:We investigate how DNA methylation absence impact transcriptionnal regulation during epiblast differentiation. More specifically, how synchronous is the naïve to primed transitioning in TKO cells compared to WT, and if there is a "germline-competent" TKO subpopulation of EpiLCs. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation WT and TKO at D0, D1, D2 and D4. We next performed single-cell SMARTseq2 RNA sequencing.
Project description:To identify the underlying mechanism causing the defects in light-dependent behavioral rhythm formation and the reduced locomotor activity in DKO and TKO zebrafish, a microarray analysis was conducted.
Project description:RNAseq was performed by to compare gene expression between wildtype and Smchd1 KO ES cells, the gene expression pattern in Dux KO mutants , Double KO mutant Tet-TKO mutants and Tet TKO plus SMCKHD1 KO mutants were analyzed by RNAseq.
