Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CDK8/19 inhibition plays an important role in pancreatic β-cell induction from human iPSCs

ABSTRACT: CDK8/19 inhibition plays an important role in pancreatic β-cell induction from human iPSCs

PROVIDER: PRJNA888999 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

CDK8/19 inhibition plays an important role in pancreatic β-cell induction from human iPSCs

Project description:In this study, we demonstrate that we can obtain human iPSC-derived pancreatic islet-like cells (iPICs) with comparable transcriptomic profile by using a combination of an ALK5 inhibitor and a CDK8/19 inhibitor, instead of utilizing ALK5 inhibitor II.

2022-12-08 | GSE215152 | GEO

ARID1A plays an important role in skin inflammation.

Project description:ARID1A plays an important role in skin inflammation.

| PRJNA966998 | ENA

Peroxidase plays important roles in soybean tolerance to Al toxicity

Project description:Peroxidase plays important roles in soybean tolerance to Al toxicity

| PRJNA480265 | ENA

MtBZR1 plays an important role in nodule development in Medicago truncatula

Project description:MtBZR1 plays an important role in nodule development in Medicago truncatula

| PRJNA545836 | ENA

Transcriptomic analysis of the effects of expression of CDK8/CDK19 and their kinase-inactive mutants in HEK293 cells (WT) and their CDK8/19 double-knockout (dKO) derivatives

Project description:RNA-Seq analysis was carried out to investigate the effects of expression of wild-type CDK8 (CDK8) or CDK19 (CDK19) or their kinase-inactive D173A mutants (CDK8M and CDK19M) in HEK293 cells (WT) and their CDK8/19 double-knockout (dKO) derivatives

2023-06-30 | GSE200753 | GEO

Gene Expresssion Analysis of Myometrium Stem Cells Treated with CDK8/19 Inhibitor

Project description:We report the gene expression changes in myometrial stem cells after 3hr treatment with CDK8/19 inhibitor (CCT251545).

2021-07-07 | GSE179494 | GEO

Effects of CDK8/19 Mediator Kinase inhibition by Senexin B on gene expression in HEK293 cells

Project description:RNA-Seq analysis was carried out to investigate the effects of selective CDK8/19 inhibitor Senexin B on gene expression in HEK293 cells (293-WT) or the CDK8/19 double-knockout (293-dKO) derivatives.

2023-06-30 | GSE221160 | GEO

CDK8/19 small molecule inhibition promotes naïve features in human and mouse pluripotent stem cells II

Project description:Pluripotent stem cells (PSCs) can transition between cell states in vitro, closely reflecting developmental changes in the early embryo. PSCs can be stabilized in their naive state by blocking extracellular differentiation stimuli, particularly FGF5 MEK signaling. Here, we report that multiple features of the naive state in human and mouse PSCs can be recapitulated without affecting FGF-MEK-signaling. Mechanistically, chemical inhibition of CDK8 and CDK19 kinases (CDK8/19i) removes their ability to repress the Mediator complex at enhancers. Thus CDK8/19i increases Mediator-driven recruitment of RNA Pol II to promoters and enhancers. This efficiently stabilizes the naive transcriptional program, and confers resistance to enhancer perturbation by BRD4 inhibition. Moreover, naive pluripotency during embryonic development coincides with reduction in CDK8/19. We conclude that global hyperactivation of enhancers drives naive pluripotency, and this can be captured in-vitro by inhibiting extracellular FGF-MEK-signaling, or downstream, by CDK8/19i. These principles may apply to other contexts of cellular plasticity.

2021-05-06 | GSE152378 | GEO

Transcriptomic analysis of the effects of CDK8 and CDK19 knockout and re-expression and selective CDK8/19 kinase inhibitor Senexin B treatment on gene expression in HEK293 cells

Project description:RNA-Seq analysis was carried out to investigate the effects of selective CDK8/19 inhibitor Senexin B (different treatment period) and knockout/re-expression of CDK8 and CDK19 proteins) on gene transcription in HEK293 cells

2022-07-01 | GSE154357 | GEO

Global hyperactivation of enhancers stabilizes human and mouse naive pluripotency through inhibition of CDK8/19 Mediator kinases

2020-02-26 | GSE127186 | GEO