Project description:Investigation of whole genome gene expression level changes in a Brucella melitensis delta prlr mutant compared to the wild type strain. The mutants analyzed in this study are further described in A. Mirabella, R-M Yanez, R.M. Delrue, S. Uzureau, M.S. Zygmunt, A. Cloeckaert, X. De Bolle, J.J. Letesson (2012). The two component system PrlS/PrlR of Brucella melitensis is required for persistence in mice and appears to respond to ionic strength. Microbiology A six chip study using total RNA recovered from three separate wild-type cultures of Brucella melitensis 16M and three separate cultures of a prlR mutant strain. Each chip measures the expression level of 3,198 genes from Brucella melitensis 16M with nineteen 60 mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.

2012-12-31 | E-GEOD-34633 | biostudies-arrayexpress

Proteomic raw data for ctrl HRECs, CAPSL knockdown HRECs, and CAPSL overexpression HRECs

Project description:The purpose of this project is to explore the changes in signaling pathways after CAPSL knockdown and overexpression, and to identify potential downstream target genes of CAPSL along with the signaling pathways they are involved in. Ultimately elucidating the molecular mechanisms underlying CAPSL pathogenesis. HRECs were transfected with ctrl shRNA, CAPSL knockdown shRNA and LentiOE CAPSL. The cells were then collected at least 72h after transfection, and samples were then usd for proteomic analysis. After cell collection, a series of cutting-edge technologies, including protein extraction, enzyme digestion, liquid chromatography-tandem mass spectrometry, and bioinformatics analysis, were employed to investigate the quantitative proteome of the samples.

2024-09-04 | PXD051696 | Pride

PrlR regulon in Brucella melitensis 16M

2012-12-31 | GSE34633 | GEO

Natural and molecular history of prolactinoma: insights from a Prlr -/- mouse model

Project description:Mouse models of prolactinoma can be useful to better understand molecular mechanisms involved in abnormal lactotroph cell proliferation and secretion. We have previously developed a prolactin receptor deficient (Prlr -/-) mouse, in which Prlr -/- females develop large secreting prolactinomas from 12 months of age with a penetrance of 100%, mimicking human aggressive densely granulated macroprolactinoma, a highly secreting subtype.

2018-02-21 | GSE100334 | GEO

Sequencing of AsPC-1 cells with CYP3A5 expression modulation

Project description:Purpose: To understand the transcriptome-wide effect of CYP3A5 knockdown or over-expression in AsPC-1 cells

2024-04-17 | GSE149000 | GEO