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Comparison of small RNA Profiles of extracellular vesicles (EVs) derived from three mesenchymal stem cell-laden Matrigel composites

ABSTRACT: Comparison of small RNA Profiles of extracellular vesicles (EVs) derived from three mesenchymal stem cell-laden Matrigel composites

PROVIDER: PRJNA889722 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21870629.fastq.gz	Fastqsanger.gz
	SRR21870630.fastq.gz	Fastqsanger.gz
	SRR21870631.fastq.gz	Fastqsanger.gz
	SRR21870632.fastq.gz	Fastqsanger.gz
	SRR21870633.fastq.gz	Fastqsanger.gz

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Comparison of small RNA Profiles of extracellular vesicles (EVs) derived from three mesenchymal stem cell-laden Matrigel composites

Project description:To investigate the difference of small RNA profiles of EVs derived from mesenchymal stem cell-laden Matrigel composites: high cell density beads (HDB) , low cell density beads (LDB) , and high cell density bulk ge (HDG)

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Crosstalk between the effects of extracellular vesicles (Evs) and TNF on U937 human monocyte cell line

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Project description:Vascular calcification often occurs with osteoporosis, a contradictory association called “calcification paradox”. We find that extracellular vesicles (EVs) released from aged bone matrix (AB-EVs) during bone resorption favor adipogenesis rather than osteogenesis of BMSCs and augment calcification of vascular smooth muscle cells (VSMCs). Intravenous or intramedullary injection of AB-EVs promotes bone-fat imbalance and exacerbates Vitamin D3 (VD3)-induced vascular calcification in young or old mice. To explore the involvement of miRNAs in the AB-EVs-induced promotion of adipocyte formation and vascular calcification, the Agilent miRNA array was conducted to compare the miRNA expression profiles in AB-EVs and YB-EVs from mouse bone specimens. Our study uncovers the role of AB-EVs as a messenger for calcification paradox by transferring functional miRNAs.

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