Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of loss of the histone lysine acetyltransferase KAT7 on mRNA levels in proliferating and differentiating mouse neural stem cells (neurosphere cells) [RNA-seq]

ABSTRACT: Effects of loss of the histone lysine acetyltransferase KAT7 on mRNA levels in proliferating and differentiating mouse neural stem cells (neurosphere cells) [RNA-seq]

PROVIDER: PRJNA891365 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21937521_1.fastq.gz	Fastqsanger.gz
	SRR21937521_2.fastq.gz	Fastqsanger.gz
	SRR21937522_1.fastq.gz	Fastqsanger.gz
	SRR21937522_2.fastq.gz	Fastqsanger.gz
	SRR21937523_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

Effects of loss of the histone lysine acetyltransferase KAT7 on mRNA levels in proliferating and differentiating mouse neural stem cells (neurosphere cells) [RNA-seq]

Project description:RNA-seq data of neural stem and progenitor cell (NSPC) isolates from Kat7lox/loxNesCreT/+ and Kat7+/+NesCreT/+ E14.5 foetuses for each, proliferating and differentiating conditions. KAT7 and H3K14ac were not required for the continued transcription of genes that were actively transcribed at the time of loss of KAT7, but indispensable for the activation of repressed genes.

2022-11-30 | GSE215970 | GEO

RNA-seq of murine proliferating neural stem and progenitor cells from Kat7-deleted mouse fetuses

Project description:Tissue-specific deletion of the gene encoding the histone acetyltransferase Kat7 (Hbo1) in the developing mouse central nervous system using cre-recombinase expression driven by regulatory sequences of the nestin locus (NesCre transgene) resulted in defective cerebral cortex development, a complete failure of neural stem cells to give rise to neurons and oligodendrocytes during in vitro differentiation, and a failure to express the neuronal differentiation program. Proliferating neural stem and progenitor cells (NPSCs) were isolated from Kat7-deleted and Kat7-intact 14.5 day embryonic mouse fetuses.

2022-12-09 | GSE220498 | GEO

miRNA Expression data from the differentiating and proliferating neural stem/progenitor cells

Project description:The adverse effect of CSDS on the DG neurogenesis is expected to affect the properties of the resident NSCs/NPCs.We used neurosphere culture system, a well-established ex vivo model to study neurogenesis. We collected proliferating neurospheres and differentiating NSCs/NPCs after 7 days of plating and isolated RNA for microRNA array to identify the altered miRNAs

2019-07-17 | GSE132822 | GEO

Gene Expression data from the differentiating and proliferating neural stem/progenitor cells

Project description:The adverse effect of CSDS on the DG neurogenesis is expected to affect the properties of the resident NSCs/NPCs.We used neurosphere culture system, a well-established ex vivo model to study neurogenesis. We collected proliferating neurospheres and differentiating NSCs/NPCs from two stages, i.e. early (after 2 days) and late (after 7 days) and isolated RNA for gene arrays.

2019-07-17 | GSE132820 | GEO

Distribution of histone H3 lysine 14 acetylation in the genome of proliferating and differentiating mouse neural stem cells (neurosphere cells) [CHIP-seq]

Project description:Anti-H3K14ac ChIP-seq data of neural stem and progenitor cell (NSPC) isolates from wild-type E14.5 foetuses for each, proliferating and differentiating conditions. H3K14ac changed during differentiation. H3K14ac levels correlated positively with the activation of the neuronal and glial gene expression programs.

2022-11-30 | GSE215971 | GEO

DNA methylation profiling of proliferating and differentiating keratinocytes

Project description:DNA methylation profiling of proliferating and differentiating keratinocytes

| PRJNA604558 | ENA

Expression profile of proliferating and differentiating primary human keratinocytes in vitro

Project description:Human epidermal keratinocytes undergo tightly controlled program of cell differentiation, leading to the formation of cornified envelope. Primary keratinocytes in vitro, under calcium stimulation mimic the differentiation program observed in vivo. Analysis of the transcription profile of two cell population, such as proliferating cells and differentiating cells helps to discover new genes implicated in that process and to understand the mechanisms of regulation of the keratinocyte differentiation. Primary human keratinocytes were cultured under proliferating (Day 0, sub-confluent cells) and differentiating (seven days of high calcium medium) conditions. As a source of cells, we used normal skin from different body sites: back, foreskin, palmoplantar. RNA extracted from cultured primary human keratinocytes were isolated from five different donors. We compared the expression profiles of proliferating versus differentiating keratinocytes.

2010-08-01 | E-GEOD-21413 | biostudies-arrayexpress

KAT7 promotes malignant phenotypes of colorectal cancer via MRAS-mediated activation of the MAPK/ERK pathway

Project description:Histone acetyltransferases (HATs) play a crucial role in transcriptional regulation by acetylating histones. Dysregulation of HAT activity is implicated in developmental disorders and cancer. In this study, we discovered an upregulated KAT7 signaling pathway in colorectal cancer (CRC) and its association with poor patient survival. Knockdown of KAT7 suppressed CRC cell viability, proliferation, migration, and invasion while promoting apoptosis. Conversely, KAT7 overexpression enhanced these cellular processes. In vivo experiments demonstrated that KAT7 knockdown inhibited CRC growth and lung metastasis. Mechanistically, KAT7 acetylated histone H3 at lysine 14 (H3K14) to enhance MRAS transcription, which activated the MAPK/ERK pathway and promoted tumorigenesis. Overexpression of MRAS or treatment with the ERK activator C6 Ceramide rescued the tumor inhibition caused by KAT7 silencing. The acetyltransferase activity of KAT7 is essential for CRC progression. Reexpression of KAT7, but not KAT7 acetyltransferase activity-deficient mutants, rescued MRAS expression, ERK phosphorylation, and CRC tumorigenesis in KAT7 knockdown CRC cells. Taken together, our results define the essential role of KAT7 in CRC tumorigenesis, rationalizing its potential as a therapeutic target for CRC treatment.

2024-06-27 | GSE270298 | GEO

Chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq) detection of histone H3 lysine 14 acetylation (H3K14ac) and KAT7 (HBO1) in E14.5 mouse neural stem and progenitor cells (NSPCs)

Project description:Tissue specific deletion of the gene encoding the histone acetyltransferase KAT7 (HBO1) in the developing mouse central nervous system using cre-recombinase expression driven by regulatory sequences of the nestin locus (NesCre transgene) resulted in defective cerebral cortex development, a complete failure of neural stem cells to give rise to neurons and oligodendrocytes during in vitro differentiation and a failure to express the neuronal differentiation program. KAT7 genome occupancy and histone H3 lysine 14 acetylation (H3K14ac) levels were assessed by ChIP-seq in proliferating Kat7 deleted vs. Kat7 intact neural stem and progenitor cells (NSPCs). KAT7 and H3K14ac were completely absent in the Kat7 deleted cells. IN KAT7 intact cells, KAT7 occupancy levels and H3K14ac levels within the body of genes correlated strongly and positively. Both were higher in genes that were expressed strongly than in genes with lower or no expression. However, KAT7 and H3K14ac were present in all genes including silent genes and in all other regions of the genome, including intergenic regions and regions decorated with histone H3 lysine 9 trimethylation (H3K9me3), an indicator of constitutive heterochromatin.

2022-11-30 | GSE216459 | GEO

meRIP-seq and RNA-seq on proliferating and differentiating cultured C2C12 cells

Project description:To determine differences in the m6A-modified transcriptional profile of cultured proliferating and differentiating myoblasts.

2020-07-21 | GSE154720 | GEO