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Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation [RNA-Seq]

ABSTRACT: Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation [RNA-Seq]

PROVIDER: PRJNA891739 | ENA |

REPOSITORIES: ENA

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Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation

Project description:Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation

| PRJNA891740 | ENA

Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation [scRNA-Seq]

Project description:Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation [scRNA-Seq]

| PRJNA891750 | ENA

Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation [scRNA-Seq]

Project description:Progress in clinical translation of gene edited porcine kidney xenotransplantation is accelerating. However, kidney function entails more than removal of metabolic waste products and it is unknown if all endocrine functions will be faithfully reproduced by porcine kidneys in primate recipients. Seventeen cynomolgus macaques with survival >60days after life sustaining kidney xenotransplantation (LSKX) from gene edited Yucatan minipigs underwent measurement of xenograft growth and two kidney dependent endocrine pathways: renin-angiotensin-aldosterone-system (RAAS) and calcium-vitamin D-parathyroid-hormone. Clinical chemistries, plasma-renin-activity, Beta-C-terminal-telopeptide (CTx) assay, RNA-seq, single-cell RNA-seq, and serial ultrasonography were conducted and generalized linear models assessed statistical relationships. Xenografts transplanted from minipigs demonstrated only modest growth (estimated ~8.0% per year) and didn’t significantly contribute to recipient RAAS pathway. However, PTH-independent hypercalcemia and hypophosphatemia were observed and might be xenograft intrinsic suggesting close monitoring and timely intervention during the first month post-transplant. Further study of these phenotypes is warranted in designing prospective clinical trials.

2023-04-25 | GSE216033 | GEO

Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation [RNA-Seq]

2023-04-25 | GSE216029 | GEO

Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-04-21 | GSE216034 | GEO

scRNA-seq of Gene-edited Porcine Kidneys Prior to Xenotransplantation into Cynomolgus Macaques

Project description:Succesful xenotransplantation of porcine kidneys into humans requires the ubiqutous expression of certain human transgenes. Modern transcriptomics now alllows gene expression to be measured at the per-cell level, which can provide important insights into the expression pattern of these life-sustaining transgenes in a complex organ such as the kidney. Analysis of this scRNA-seq data shows ubiquitous expression of transgenes in most major kidney cell populations which includes various subtypes of endothelial cells.

2023-04-21 | GSE210557 | GEO

Serial xenotransplantation in NSG mice promotes a hybrid epithelial/mesenchymal gene expression signature and stemness in rhabdomyosarcoma cells

Project description:NSTS-11 cell line derived from a primary embryonal rhabdomyosarcoma was used for serial xenotransplantation in NSG mice in vivo. Subsequently, three cell lines, LTB1, LTB5, and LTB24, were derived from primary, secondary, and tertiary xenograft tumors, respectively. Gene expression profiling of all four cell lines was performed to identify changes in gene expression over the course of serial xenotransplantation, which was associated with a gradual increase of stemness of embryonal rhabdomyosarcoma cells. For each of the xenograft-derived cell lines, the parental NSTS-11 cell line served as a reference control.

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Cellular dynamics of pig-to-human kdiney xenotransplantation [RNA-seq]

Project description:We performed comprehensive single-cell RNA sequencing (scRNA-seq) analyses of the two porcine xenografts and their contralateral untransplanted kidneys to dissect xenotransplantation-associated cellular dynamics and xenograft-recipient interactions. We additionally performed longitudinal bulk RNA-seq on core biopsies to dissect the time-resolved physiological changes of the xenografts and longitudinal scRNA-seq of peripheral blood mononuclear cells (PBMCs) to detect recipient’s immune responses across time.

2024-06-30 | GSE257541 | GEO

SCD1 Modulates the Skin Homeostasis via Sustaining Hemidesmosomes

Project description:SCD1 Modulates the Skin Homeostasis via Sustaining Hemidesmosomes

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Dynamics in protein translation sustaining T cell preparedness

Project description:Dynamics in protein translation sustaining T cell preparedness

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