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ABSTRACT: Mitomycin C-induced effects on soil methanotrophs (pmoA data)

PROVIDER: PRJNA896971 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22143600_1.fastq.gz	Fastqsanger.gz
	SRR22143600_2.fastq.gz	Fastqsanger.gz
	SRR22143601_1.fastq.gz	Fastqsanger.gz
	SRR22143601_2.fastq.gz	Fastqsanger.gz
	SRR22143602_1.fastq.gz	Fastqsanger.gz

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Project description:Mitomycin C-induced effects on soil methanotrophs

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Biomarker Response of Aerobic Methanotrophs to Methane Oxidation Rates and Short-Term Methane and Oxygen Limitation

Project description:Methanotrophs, which help regulate atmospheric levels of methane, are active in diverse natural and man-made environments. This range of habitats and the feast-famine cycles seen by many environmental methanotrophs suggest that methanotrophs dynamically mediate rates of methane oxidation. Global methane budgets require ways to account for this variability in time and space. Functional gene biomarker transcripts are increasingly being studied to inform the dynamics of diverse biogeochemical cycles. Previously, per-cell transcript levels of the methane oxidation biomarker, pmoA, were found to vary quantitatively with respect to methane oxidation rates in model aerobic methanotroph, Methylosinus trichosporium OB3b. In the present study, these trends were explored for two additional aerobic methanotroph pure cultures, Methylocystis parvus OBBP and Methylomicrobium album BG8. At steady-state conditions, per cell pmoA mRNA transcript levels strongly correlated with per cell methane oxidation across the three methanotrophs across many orders of magnitude of activity (R2 = 0.91). Additionally, genome-wide expression data (RNA-seq) were used to explore transcriptomic responses of steady state M. album BG8 cultures to short-term CH4 and O2 limitation. These limitations induced regulation of genes involved in central carbon metabolism (including carbon storage), cell motility, and stress response.

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2013-09-28 | E-GEOD-51235 | biostudies-arrayexpress

Project description:soil pmoA metagenone

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ChIP-seq of Mycobacterium smegmatis PafBC to identify in vivo binding sites

Project description:We aimed to determine the binding sites of the putative transcriptional regulator PafBC under DNA damage stress induced by mitomycin C or under oxidative stress induced by hydrogen peroxide. Therefore, we chose a ChIP-seq approach, crosslinking cells before PafBC-DNA complexes were immunoprecipitated with a PafBC-specific antibody.

2018-06-17 | E-MTAB-6503 | biostudies-arrayexpress

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