Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ptch1/Shh signaling control cochlear marginal cell differentiation and stria vascularis formation

ABSTRACT: Ptch1/Shh signaling control cochlear marginal cell differentiation and stria vascularis formation

PROVIDER: PRJNA898638 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Ptch1/Shh signaling control cochlear marginal cell differentiation and stria vascularis formation

Project description:The stria vascularis (SV) is an intricate non-sensory structure composed of multiple cell layers of different origins in the mammalian cochlea. It generates and maintains the endolymph, and therefore is essential for hearing functions. Here, using single cell transcriptomics and transgenic mouse models, we discover that the Shh receptor Ptch1 is required for marginal cell (MC, the epithelial cell layer of SV) differentiation and SV development. During cochlear development, MC differentiation gradually proceeds from cochlear base to apex, resembling the spatiotemporal differentiation pattern of sensory hair cells. Inactivation of Ptch1 leads to an impaired MC differentiation through elevated Gli2 levels, and inhibition of Gli2/Shh signaling activity is a prerequisite for MC differentiation. Single cell transcriptome analyses reveal that MCs are specified as early as E14. Moreover, in the absence of Ptch1, MC precursors are maintained in the progenitor state and differentiation is blocked consequently.

2024-04-03 | GSE217373 | GEO

RNA-seq of porcine inner ear stria vascularis

Project description:To clarify the effect of Mitf mutation on stria vascularis transcriptome and identify downstream-genes of Mitf pathway which responsible for hearing development, we employed high-throughput sequencing to analyze the transcriptome of MitfR/r and Mitfr/r stria vascularis.

2016-05-31 | GSE80665 | GEO

RNA-seq of porcine inner ear stria vascularis

2016-05-31 | E-GEOD-80665 | biostudies-arrayexpress

Transcription profiling of mouse stria vascularis FROM 129S6 strain lacking slc26a4 AND wildtype controls

Project description:To see for differential expression of genes in the stria vascularis of wild type and pendrin knockout mice Experiment Overall Design: Six samples in total of stria vascularis RNA were analyzed. Triplicates from wild type and pendrin knockout mice were run and analyzed.

2008-04-07 | E-GEOD-4749 | biostudies-arrayexpress

Transcription profiling of mouse with ablation of connexin 30 in the stria vascularis

Project description:Effect of the ablation of connexin 30 in the stria vascularis

2007-04-16 | E-MEXP-558 | biostudies-arrayexpress

Project description:Age-related hearing loss is a progressive sensorineural hearing loss that occurs as people get older. Degeneration of the organ of Corti and atrophy of the lateral wall of the scala media in the inner ear are the two primary causes. Transcriptome analysis has emerged as a powerful tool in revealing the genetic and molecular profile of a cell or a population of cells during aging in organism models, animals and humans. Comparison of transcriptomes between young and older animals have identified many common genes and pathways that are associated with aging and longevity. We used RNA-seq to examine changes in gene expression profiles of the stria vascularis between 9- and 26-month-old CBA mice. Our transcriptome analysis provides a rich resource for mechanistic studies of biological aging of stria vascularis.

2022-03-01 | GSE154833 | GEO

Transcription profiling of mouse stria vascularis from animals lacking Slc26a4 and heterzygous controls before the onset of hearing.

Project description:Determination of differential expression of genes in the stria vascularis of pendrin (Slc26a4) heterozygous and knockout mice before the onset of hearing at postnatal day 10 (P10). Experiment Overall Design: A total of Six samples of stria vascularis RNA obtained from P10 mice were analyzed. Triplicates from pendrin (Slc26a4) heterozygous and knockout mice were run and analyzed.

2009-03-01 | E-GEOD-10587 | biostudies-arrayexpress

Microarray based comparison of gene expression in stria vascularis of 129S6 mice lacking slc26a4 and wildtype controls

Project description:To see for differential expression of genes in the stria vascularis of wild type and pendrin knockout mice Keywords: Knockout vs Control

2008-02-20 | GSE4749 | GEO

Characterizing cellular heterogeneity and homeostatic gene regulatory networks in the adult mammalian stria vascularis using single cell and single nucleus transcriptional profiling

Project description:Characterzing adult mouse stria vascularis heterogeneity and gene regulatory networks

2019-12-10 | GSE136196 | GEO

Gene expression in stria vascularis of mice lacking Slc26a4 and heterzygous controls before the onset of hearing.

2009-02-19 | GSE10587 | GEO