Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIP-seq analysis of E2FA, E2FB, E2FC and RBR targets

ABSTRACT: ChIP-seq analysis of E2FA, E2FB, E2FC and RBR targets

PROVIDER: PRJNA903916 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22362214.fastq.gz	Fastqsanger.gz
	SRR22362215.fastq.gz	Fastqsanger.gz
	SRR22362216.fastq.gz	Fastqsanger.gz
	SRR22362217.fastq.gz	Fastqsanger.gz
	SRR25098606.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 7

Similar Datasets

Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha

Project description:Estrogen Receptor alpha (ERα) is a key driver of most breast cancers, and it is the target of endocrine therapies used in the clinic to treat women with ERα positive (ER+) breast cancer. The two methods ChIP-seq (chromatin immunoprecipitation coupled with deep sequencing) and RIME (Rapid Immunoprecipitation of Endogenous Proteins) have greatly improved our understanding of ERα function during breast cancer progression and in response to anti-estrogens. A critical component of both ChIP-seq and RIME protocols is the antibody that is used to pull down the bait protein. To date, most of the ChIP-seq and RIME experiments for the study of ERα have been performed using the sc-543 antibody from Santa Cruz Biotechnology. However, this antibody has been discontinued, thereby severely impacting the study of ERα in normal physiology as well as diseases such as breast cancer and ovarian cancer. Here, we compare the sc-543 antibody with other commercially available antibodies, and we show that 06-935 (EMD Millipore) and ab3575 (Abcam) antibodies can successfully replace the sc-543 antibody for ChIP-seq and RIME experiments.

2019-03-21 | PXD012930 | Pride

Identifying transcriptional targets of ONECUT3 by ChIP-seq

Project description:Identifying transcriptional targets of ONECUT3 by ChIP-seq

| PRJNA836928 | ENA

ZFP541 ChIP-seq targets during male meiotic prophase

Project description:ZFP541 ChIP-seq targets during male meiotic prophase

| PRJNA688209 | ENA

Global analysis of enhancer targets: Mosaic-seq

Project description:Global analysis of enhancer targets: Mosaic-seq

| PRJNA532919 | ENA

Florigen family chromatin recruitment, competition and targets [ChIP-Seq]

Project description:Florigen family chromatin recruitment, competition and targets [ChIP-Seq]

| PRJNA531668 | ENA

The identification of Dwg downstream targets using ChIP-seq

Project description:The identification of Dwg downstream targets using ChIP-seq

| PRJNA911806 | ENA

ChIP-seq analysis of IMR32

Project description:ChIP-seq analysis of IMR32

| PRJNA862179 | ENA

ChIP-seq analysis reveals the direct targets of GltR in the P. aeruginosa genome

Project description:ChIP-seq analysis reveals the direct targets of GltR in the P. aeruginosa genome

| PRJNA644345 | ENA

Identification of BCL11A targets by CHIP Seq

Project description:We have recently identified BCL11A as a key regulator of mammary stem (MaSC) and progenitor cells. Deletion of BCL11A in MaSC leads to loss of ability of those cells to engraft a cleared mammary fatpad. This biological function of BCL11A correlates with its overexpression in basal breast cancers which are thought to arise from stem and progenitor subtypes. Basal breast cancer cell lines where BCL11A is knocked down using shRNA failed to develop tumours in xenograft transplantations in mice. Therefore, BCL11A is potentially a novel target for breast cancer treatment. Microarray analysis has identified potential gene targets for BCL11A however, it is not known if these targets are directly regulated by BCL11A at the transcription level. We would like to perform CHIP-SEQ analysis on a mammary cell line using BCL11A antibody under two conditions (normal expression and overexpression of BCL11A).This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/protocol to be provided

2013-01-28 | E-ERAD-69 | biostudies-arrayexpress

ChIP-AP - An Integrated ChIP-Seq Analysis Pipeline

Project description:ChIP-AP - An Integrated ChIP-Seq Analysis Pipeline

| PRJNA723011 | ENA