Project description:Needle biopsies were obtained from the vastus lateralis muscle of 6 healthy, sedentary, 67±2.5 year-old males before and after 3 months of training. Keywords: other
Project description:Needle biopsies were obtained from the vastus lateralis muscle of 6 healthy, sedentary, 67 +/- 2.5 year-old males before and after 3 months of training.
Project description:Proteomic characterization of skeletal muscle biopsies collected from the vastus lateralis of 44 male and female human subjects. Research subjects were divided in three different groups based on their individual exercise backgrounds and physical performance testing: 1) endurance trained (males; ME, n = 9 and females; FE, n = 9, with at least 15 years’ of regular training experience), 2) strength-trained males (MS, n = 9, with at least 15 years’ of regular training experience), and 3) age-matched healthy untrained controls (males, MC, n = 9 and females FC, n = 8, with a self-reported history of <2 exercise bouts per week over the past 15 years).
Project description:Recreationally-trained college aged males underwent 10 weeks of moderate volume, high-load resistance exercise with PRE and POST vastus lateralis skeletal muscle biopsies. Protein isolation was performed on tissue samples and the sarcoplasmic protein fraction was analyzed to evaluate changes PRE to POST.
Project description:We sequenced mRNA in vastus lateralis muscle samples from two amateur endurance-trained males (V’O2max 57 ml/min/kg) before and after acute endurance exercise
Project description:Aging is associated with mitochondrial dysfunction and insulin resistance. We conducted a study to determine the role of long-term vigorous endurance exercise on age-related changes in insulin sensitivity and various indices of mitochondrial functions. Experiment Overall Design: Skeletal muscle transcript profiling was done using Vastus Lateralis muscle biopsy samples from 10 young sedentary (YS), 10 older sedentary (OS), 10 young trained (YT) and 10 older trained (OT) men and women. Note that YT2, YS1, and OT1 didn't pass the Quality Control Step of dChip (high array/single outliers). Sedentary subjects exercised less than 30 min/day, twice per week. Trained subjects performed ⥠1 hour cycling or running 6 days/week over the past 4 years.
Project description:vastus lateralis biopsies were obtained from healthy subjects, either young adults (20-29 yr old) or older (65-75 yr old) Keywords = muscle Keywords = aging Keywords: other
Project description:Global microarray (HG U133 Plus 2.0) was used to investigate the effects of resistance exercise and resistance training on the skeletal muscle transcriptome profile of 28 young and old adults. Vastus lateralis muscle biopsies were obtained pre and 4hrs post resistance exercise in the beginning (untrained state) and at the end (trained state) of a 12 wk progressive resistance training program. A total of 28 subjects were included in this investigation. The young (n=16, 24±1y) participants included 8 males and 8 females. The old (n=12, 84±1y) participants included 6 males and 6 females. All subjects participated in 12 wks of progressive resistance training consisting of bilateral knee extensions with 3x10 reps at 70% of 1-RM, and 3d/wk for a total of 36 training sessions. Vastus lateralis biopsies were obtained in conjunction with the 1st and 36th (last) training session and included a basal biopsy and another biopsy 4hrs post the resistance exercise session (for a total of 4 biopsies per subject). The RNA integrity for all samples was very good with an average RIN number of 7.6. All 110 samples were analyzed on a separate microarray chip, and samples were not pooled between subjects. The study design allowed us to examine the acute effects of resistance exercise on the skeletal muscle transcriptome in the untrained and trained state. The design also provided information on the effects of resistance training on basal level gene expression and the effects of age on basal level gene expression
Project description:The study used liquid-chromatography tandem mass spectrometry (LC-MS/MS) combined with bioinformatics analysis to analyze muscle biopsies from the vastus lateralis muscles obtained from two groups of young males after four weeks of either concentric or eccentric resistance training of the quadriceps femoris muscles.
Project description:RNA was pooled from vastus lateralis biopsies obtained from 8 younger (21-24 yr) and 8 older (66-77 yr)men. Healthy subjects with no neuromuscular disease. Keywords: other