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Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids

ABSTRACT: Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids

PROVIDER: PRJNA906588 | ENA |

REPOSITORIES: ENA

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Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)

Project description:Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)

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Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (scRNA-Seq)

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Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (scRNA-Seq)

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Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)

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Chemically-defined and scalable culture system for intestinal stem cells derived from human intestinal organoids.

Project description:Three-dimensional human intestinal organoids (hIO) are widely used as a platform for biological and biomedical research. However, reproducibility and challenges for large-scale expansion limit their applicability. Here, we establish a human intestinal stem cell (ISC) culture method expanded under feeder-free and fully defined conditions through selective enrichment of ISC populations (ISC3D-hIO) within hIO derived from human pluripotent stem cells. The intrinsic self-organisation property of ISC3D-hIO, combined with air-liquid interface culture in a minimally defined medium, forces ISC3D-hIO to differentiate into the intestinal epithelium with cellular diversity, villus-like structure, and barrier integrity. Notably, ISC3D-hIO is an ideal cell source for gene editing to study ISC biology and transplantation for intestinal diseases. We demonstrate the intestinal epithelium differentiated from ISC3D-hIO as a model system to study severe acute respiratory syndrome coronavirus 2 viral infection. ISC3D-hIO culture technology provides a biological tool for use in regenerative medicine and disease modelling.

| S-EPMC10821882 | biostudies-literature

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-11-20 | GSE219018 | GEO

Xeno-free and Chemically Defined Human System for Culturing Human Epidermal Keratinocytes

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Transcription profiling of human embryonic stem cells and their differentiated progeny grown in chemically defined conditions

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Transcription profling of human embryonic stem cells differentiated into endoderm progenitors in chemically-defined media.

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Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector Production.

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