Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)

ABSTRACT: Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)

PROVIDER: PRJNA906590 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22447135_1.fastq.gz	Fastqsanger.gz
	SRR22447135_2.fastq.gz	Fastqsanger.gz
	SRR22447136_1.fastq.gz	Fastqsanger.gz
	SRR22447136_2.fastq.gz	Fastqsanger.gz
	SRR22447137_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids

Project description:Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids

| PRJNA906588 | ENA

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (scRNA-Seq)

Project description:Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (scRNA-Seq)

| PRJNA906591 | ENA

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (scRNA-Seq)

Project description:we established a human intestinal stem cell (ISC) culture method expanded under feeder-free and fully defined conditions through selective enrichment of ISC populations (ISC3D-hIO) within hIO derived from human pluripotent stem cells.

2023-11-20 | GSE219017 | GEO

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)

2023-11-20 | GSE219016 | GEO

Chemically-defined and scalable culture system for intestinal stem cells derived from human intestinal organoids.

Project description:Three-dimensional human intestinal organoids (hIO) are widely used as a platform for biological and biomedical research. However, reproducibility and challenges for large-scale expansion limit their applicability. Here, we establish a human intestinal stem cell (ISC) culture method expanded under feeder-free and fully defined conditions through selective enrichment of ISC populations (ISC3D-hIO) within hIO derived from human pluripotent stem cells. The intrinsic self-organisation property of ISC3D-hIO, combined with air-liquid interface culture in a minimally defined medium, forces ISC3D-hIO to differentiate into the intestinal epithelium with cellular diversity, villus-like structure, and barrier integrity. Notably, ISC3D-hIO is an ideal cell source for gene editing to study ISC biology and transplantation for intestinal diseases. We demonstrate the intestinal epithelium differentiated from ISC3D-hIO as a model system to study severe acute respiratory syndrome coronavirus 2 viral infection. ISC3D-hIO culture technology provides a biological tool for use in regenerative medicine and disease modelling.

| S-EPMC10821882 | biostudies-literature

Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-11-20 | GSE219018 | GEO

Xenogeneic-free culture of human intestinal stem cells on functional polymer-coated substrates for scalable, clinical-grade stem cell therapy

Project description:Inevitable need for basement membrane extract (BME) with undefined constituents, such as Matrigel, for intestinal stem cell (ISC) culture in traditional system poses a significant barrier that must be overcome for the development of clinical-grade, scalable, ready-to-use ISCs. Here, we propose a functional polymer-based xenogeneic-free dish for the culture of intestinal stem cells (XF-DISC), ensuring substantially prolonged maintenance of ISCs derived from 3-dimensional human intestinal organoid (ISCs3D-hIO). XF-DISC enables remarkable expandability, exhibiting a 24-fold amplification in cell number within 30 days, with long-term maintenance of ISCs3D-hIO through consecutive passaging more than 30 times (> 210 days), and is fully compatible with a cell banking system. Notably, the ISCs3D-hIO cultured on XF-DISC are successfully transplanted into the intestinal injury and inflammation models, leading to full regeneration of damaged intestinal epithelium. XF-DISC, an extraordinary reliable and scalable xenogeneic-free ISC3D-hIO culture method exhibits significant promise in the development of regenerative ISC therapy for human intestinal diseases.

2024-10-22 | PXD050747 | Pride

Xeno-free and Chemically Defined Human System for Culturing Human Epidermal Keratinocytes

Project description:Xeno-free and Chemically Defined Human System for Culturing Human Epidermal Keratinocytes

| PRJNA431596 | ENA

Transcription profiling of human embryonic stem cells and their differentiated progeny grown in chemically defined conditions

Project description:Comparison of H9 human embryonic stem cells, grown in chemically defined and animal-product free conditions, with their differentiated progeny - mesendodermal progenitors arisen as the result of changes made to chemically defined hESC growth conditions. Contributes to a larger work on the use of chemically defined growth conditions to direct hESC differentiation.

2009-07-31 | E-MEXP-930 | biostudies-arrayexpress

Xenogeneic-free culture of human intestinal stem cells on functional polymer-coated substrates for scalable, clinical-grade stem cell therapy

2024-10-21 | GSE261785 | GEO