Project description:Series includes pooled (n = 5 mice per biological replicate) samples from submandibular, sublingual, parotid, lacrimal, and meibomian glands of BALB/c mice. Both male and female samples were analyzed on CodeLink Mouse Uniset I Microarrays. Keywords: repeat sample
Project description:Series includes pooled (n = 5 mice per biological replicate) samples from lacrimal, meibomian, and submandibular glands of male palcebo- and testosterone-treated BALB/c mice. All experiments were run in triplicate (pooled biological replicates) on CodeLink Mouse Uniset I Microarrays. Keywords: repeat sample
Project description:Our objective was to determine the nature and extent of androgen regulation of gene expression in the female lacrimal, meibomian,and submandibular glands, and to explore the degree to which this control is the same as in male glands. Keywords: Hormone treatment
Project description:The aim of this study is characterize the gene expression of rat parotid, submandibular and sublingual glands, providing basic information for the salivary marker proteins.
Project description:The Effect of Aromatase Knockout on Gene Expression in the Mouse Lacrimal and Meibomoan Gland. Keywords: Aromatase Knockout vs Wild Type Control Lacrimal and meibomian glands were harvested from homozygous male and female aromatase knockout mice and age matched wild type controls. Tissues were pooled into 3 biological replicates and were hybridized to separate microarrays. Each cRNA prep was hybridized to a GE Healthcare/Amersham Biosciences CodeLink UniSet Mouse 20K I Bioarray and a Affymetrix GeneChip Mouse Expression Array 430A.
Project description:Dried saliva spot (DSS) sampling is a minimally invasive extraction technique that allows for the spatial mapping of salivary protein distribution in the oral cavity. In conjunction with untargeted nanoLC-Orbitrap-MS/MS analysis, we used DSS to compare the unstimulated proteomes secreted by the parotid and submandibular/sublingual salivary glands
Project description:This laboratory studies the structural basis of carbohydrate function in terms of adhesion and other biological processes. The Fisher lab is testing the hypothesis that the mucin-coated oral and uterine cavities present similar carbohydrate receptors that specify the bacterial ecology of both regions and the repertoire of these oligosaccharide species is hormonally regulated. This theory also suggests that certain individuals express carbohydrate receptors that make them susceptible to both periodontal disease and preterm labor. Experimental procedure: 40 mature female mice were ovariectomize . The mice were allowed to rest for two weeks to eliminate any remaining endogenous estrogen (E2) and progesterone (P4). Then the mice were separated into four groups (10 each) that received the treatment indicated. Group 1: ovariectomized mice with no supplement, only sesame oil vehicle. Group 2: ovariectomized mice with only P4 supplement (2 mg/day/mouse). Group 3: ovariectomized mice with E2 supplement (100 ng/day/mouse). Group 4: ovariectomized mice with both P4 (2 mg/day/mouse) and E2 (100 ng/day/mouse) supplement. The treatment lasted for 4 days; the steroids were dissolved in sesame oil and injected subcutaneously. After treatment, salivary glands (parotid, submandibular, and sublingual gland), submandibular lymph nodes and uterine horns with cervix were collected. Total RNA was extracted from these samples using Trizol.
Project description:Prior to the onset of autoimmune destruction, type 1 diabetic patients and an animal model thereof, the nonobese diabetic (NOD) mouse, show morphological and functional abnormalities in target organs, which may act as inciting events for leukocyte infiltration. To better understand these abnormalities, but without the complications associated with inflammatory infiltrates, we examined genes expressed in autoimmune target tissues (pancreas, submandibular glands, and lacrimal glands) of NOD/scid mice and of autoimmune-resistant C57BL6/scid mice. Experiment Overall Design: Pancreata (6 weeks old mice), submandibular (9 and 15 weeks), and lacrimal glands (15 weeks) from individual NOD-scid and B6-scid mice were isolated for RNA extraction and hybridization on Affymetrix microarrays.
