Expression data of islets between 3- and 7- day old pigs
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA909307 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The yield, cell composition, and function of islets isolated from various ages of neonatal pigs were characterized using in vitro and in vivo experimental models. Islets from 7- and 10-day old pigs showed significantly better function both in vitro and in vivo compared to islets from 3- and 5-day old pigs however, the islet yield from 10-day old pigs were significantly less than those obtained from the other pigs. Since islets from 3-day old pigs were used in our previous studies and islets from 7-day old pigs reversed diabetes more efficiently than islets from other groups, we further evaluated the function of these islets post-transplantation. B6 rag-/- mouse recipients of various numbers of islets from 7-day old pigs achieved normoglycemia faster and showed significantly improved response to glucose challenge compared to the recipients of the same numbers of islets from 3-dayold pigs. These results are in line with the findings that islets from 7-day-old pigs showed reduced voltage-dependent K+ (Kv) channel activity and their ability to recover from post-hypoxia/reoxygenation stress. Despite more resident immune cells and immunogenic characteristics detected in islets from 7-day old pigs compared to islets from 3-day old pigs, the combination of anti-LFA-1 and anti- CD154 monoclonal antibodies are equally effective at preventing the rejection of islets from both age groups of pigs. Collectively, these results suggest that islets from various ages of neonatal pigs vary in yield, cellular composition, and function. Such parameters may be considered when defining the optimal pancreas donor for islet xenotransplantation studies.
2022-12-09 | GSE220256 | GEO
Project description:Comparison between PRRS and control in female and male pig amygdala in 22 day old pigs
| PRJNA629765 | ENA
Project description:Maternal immune activation was conducted in pregnant gilt receiving either PRRS or control and measure gene expression at day 22 after birth in female and male pigs.
2020-08-28 | GSE149695 | GEO
Project description:Islets from 6-week old NOR and NOD mice were submitted to proteomics analysis to study differences in insulitis and type 1 diabetes development.
2024-05-21 | PXD021475 | Pride
Project description:From gestation day 75 to gestation day 90, an important stage for the placental and fetal development, the fetuses grow rapidly and need adequate nutrition. The Meishan pigs and the Large White pigs employ different ways in supplying the enough nutrients and oxygen to the fetus. The Meishan pigs increased the vascular density and the Large White pigs have the second increase in the surface of placenta. To understand the molecular basis related to late gestation placenta development in Chinese indigenous and Western breeds with different placental efficiency, samples were collected and used to hybridized. The results offered new data on understanding the molecular basis of placenta efficiency, and indicated that Erhualian pigs had the more efficient than the Large White pigs.
2009-06-20 | GSE11853 | GEO
Project description:To identify a physiologic postanal transcriptomic program between postnatal day 20 and 60, we first analyzed the gene expression profile in 60 day-old ( young adult) wild-type mice (WT) and compared it to 20 day-old WT mice To identify the gene expression between postnatal day 20 and 60 under the strict dependence of cardiac ephrin-B1, we compared gene expression in 20 day-old and 60 day-old Efnb1 CMspe KO (a-MHC-Cre-/+ Efnb1 flox/flox or) to 20 day-old and 60 day-old WT mice
2023-01-06 | GSE196257 | GEO
Project description:Hezuo pigs are known in China for early sexual maturity. In this study, we obtained transcriptome data from testicular tissues of 30-day-old and 120-day-old Hezuo and Landrace pigs. Through comparative analysis of their differences, we searched for some genes related to the regulation of precocious sexual traits in male cooperating pigs and revealed their molecular regulatory mechanisms, which can then provide references for the diagnosis and treatment of precocious sexual diseases in humans and for accelerating the breeding of high-fertility animals.
2021-08-31 | GSE171756 | GEO
Project description:Hezuo pigs are known in China for early sexual maturity. In this study, we obtained small RNA-Seq data from testicular tissues of 30-day-old and 120-day-old Hezuo and Landrace pigs. Through comparative analysis of their differences, we searched for some miRNAs related to the regulation of precocious sexual traits in male cooperating pigs and revealed their molecular regulatory mechanisms, which can then provide references for the diagnosis and treatment of precocious sexual diseases in humans and for accelerating the breeding of high-fertility animals.
2022-11-18 | GSE192462 | GEO
Project description:To investigate effects of Adjudin on gene expression of postnatal day 0 mouse islets (P0 islets), islets from postnatal day 0 mouse (regardless of sex) were isolated, cultured in incubator for overnight recovery, and treated with either DMSO or 10 µM Adjudin for 1 day before RNA sequencing.
2023-07-07 | GSE235397 | GEO
Project description:From gestation day 75 to gestation day 90, an important stage for the placental and fetal development, the fetuses grow rapidly and need adequate nutrition. The Meishan pigs and the Large White pigs employ different ways in supplying the enough nutrients and oxygen to the fetus. The Meishan pigs increased the vascular density and the Large White pigs have the second increase in the surface of placenta. To understand the molecular basis related to late gestation placenta development in Chinese indigenous and Western breeds with different placental efficiency, samples were collected and used to hybridized. The results offered new data on understanding the molecular basis of placenta efficiency, and indicated that Erhualian pigs had the more efficient than the Large White pigs. Experiment Overall Design: Placenta efficiency (the body weight of a piglet divided by the mass of its placenta) of Erhualian pigs is markedly higher than Large White pigs. so placenta samples (female) from 6 Erhuanlian pregnant gilts at gestation day 75 (E75) and day 90 (E90) and 6 Large White pregnant gilts at gestation day 75 (L75) and day 90 (L90) were collected. RNAs from two female piglet placentas from each gilt were combined to 12 pools and hybridized to the porcine Affymetrix GeneChip.
2009-07-01 | E-GEOD-11853 | biostudies-arrayexpress