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Decoding Reciprocal Regulation of ASPM and FoxM1 Amplifies the Oncogenic Progression in Human Hepatocellular Carcinoma Protein Composition of Whole Nucleosomes with Nuc-MS

ABSTRACT: Decoding Reciprocal Regulation of ASPM and FoxM1 Amplifies the Oncogenic Progression in Human Hepatocellular Carcinoma Protein Composition of Whole Nucleosomes with Nuc-MS

PROVIDER: PRJNA910584 | ENA |

REPOSITORIES: ENA

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Decoding Reciprocal Regulation of ASPM and FoxM1 Amplifies the Oncogenic Progression in Human Hepatocellular Carcinoma Protein Composition of Whole Nucleosomes with Nuc-MS

Project description:Fork-head box protein M1 (FoxM1) is a transcription factor which plays critical roles in development and progression of multiple cancers, including human hepatocellular carcinoma (HCC). However, the regulatory mechanism of FoxM1 itself remains largely unclear. Here, we discovered a novel role of abnormal spindle-like microcephaly associated (ASPM) in HCC, which binds to FoxM1 protein and enhances its stability by preventing proteasome-mediated degradation. ChIP-sequencing data showed that ASPM and FoxM1 co-occupies on the promoter region of multiple genes to promote their transcriptions, leading to the enhancement of a FoxM1-driven oncogenic progression. Intriguingly, FoxM1 binds to the promoter region of ASPM and transcriptionally activates the ASPM expression. Furthermore, data from clinical samples showed that a higher co-expression of ASPM and FoxM1 significantly correlates with poor prognosis in HCC, indicating that a positive feedforward loop between ASPM and FoxM1 coordinately promotes the aggressive progression in HCC. Collectively, our study reveals that reciprocal regulation of ASPM and FoxM1 amplifies the oncogenic progression and emphasizes an ASPM-FoxM1 feedforward loop could be a potential biomarker and therapeutic target in HCC.

2024-09-16 | GSE220649 | GEO

Reciprocal Regulation of ASPM and FoxM1

2024-11-01 | GSE218100 | GEO

Reciprocal Regulation of ASPM and FoxM1

Project description:Reciprocal Regulation of ASPM and FoxM1

| PRJNA902339 | ENA

Decoding the protein composition of whole nucleosomes with Nuc-MS

Project description:Current proteomic approaches disassemble and digest nucleosome particles, blurring readouts of the ‘histone code’. To preserve nucleosome-level information, we developed Nuc-MS, which displays the landscape of histone variants and their post-translational modifications (PTMs) in a single mass spectrum. Combined with immunoprecipitation, Nuc-MS quantified nucleosome co-occupancy of histone H3.3 with variant H2A.Z (sixfold over bulk) and the co-occurrence of oncogenic H3.3K27M with euchromatic marks (for example, a >15-fold enrichment of dimethylated H3K79me2). Nuc-MS is highly concordant with chromatin immunoprecipitation-sequencing (ChIP-seq) and offers a new readout of nucleosome-level biology.

2021-04-20 | GSE149484 | GEO

Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS

2022-11-03 | GSE216732 | GEO

Decoding the protein composition of whole nucleosomes with Nuc-MS

Project description:Nuc-MS characterizes histone modifications and variants directly from intact endogenous mono-nucleosomes. Preserving whole nucleosome particles enables precise interrogation of their protein content, as for H3.3-containing nucleosomes which had 6-fold co-enrichment of variant H2A.Z over bulk chromatin. Moreover, Nuc-MS showed co-occurrence of oncogenic H3.3K27M with euchromatic marks (e.g., H4K16ac and >40-fold enrichment of H3K79me2). By capturing the entire epigenetic landscape, Nuc-MS provides a new, quantitative readout of nucleosome-level biology.

2020-04-06 | MSV000085238 | MassIVE

Decoding the protein composition of whole nucleosomes with Nuc-MS

Project description:Decoding the protein composition of whole nucleosomes with Nuc-MS

| PRJNA628862 | ENA

Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS

Project description:Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS

| PRJNA895070 | ENA

Single-cell RNA-seq of ASPM mutant ferret embryonic forebrain

Project description:As described in our paper "Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size" (Johnson et al., Nature 2018), we used the standard Drop-seq method and analysis of Macosko et al. (2015) to capture, sequence, and analyze mRNA from single cells from Aspm wild-type, heterozygous, and knock-out littermate ferrets at embryonic day 35. Bulk reference samples were processed using standard Illumina mRNA-seq library prep and sequencing protocols, and the samples were described previously (Johnson, Wang et al., Nature Neurosci 2015)

2018-04-18 | GSE110010 | GEO

Decoding the protein composition of whole nucleosomes with Nuc-MS.

Project description:Current proteomic approaches disassemble and digest nucleosome particles, blurring readouts of the 'histone code'. To preserve nucleosome-level information, we developed Nuc-MS, which displays the landscape of histone variants and their post-translational modifications (PTMs) in a single mass spectrum. Combined with immunoprecipitation, Nuc-MS quantified nucleosome co-occupancy of histone H3.3 with variant H2A.Z (sixfold over bulk) and the co-occurrence of oncogenic H3.3K27M with euchromatic marks (for example, a >15-fold enrichment of dimethylated H3K79me2). Nuc-MS is highly concordant with chromatin immunoprecipitation-sequencing (ChIP-seq) and offers a new readout of nucleosome-level biology.

| S-EPMC7954958 | biostudies-literature

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