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Verson’s glands contribute to molting and antimicrobial response in the Fall armyworm

ABSTRACT: Verson’s glands contribute to molting and antimicrobial response in the Fall armyworm

PROVIDER: PRJNA912203 | ENA |

REPOSITORIES: ENA

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Verson’s glands contribute to molting and antimicrobial response in the Fall armyworm

Project description:Verson’s glands are segmental pairs of dermal glands attached to the epidermis in lepidopteran larvae. They produce macromolecules during intermolt period and empty them during each molt. Morphological, histochemical, developmental, and protein analysis studies have been conducted to determine the functions of Verson’s glands. However, the exact role of Verson’s glands remains unclear. In our previous study, a strain of transgenic fall armyworm, Spdoptera frugiperda expressing green fluorescence protein (GFP) and Systemic RNA interference defective protein 1 (SID-1) from Caenorhabditis elegans was established to improve RNA interference (RNAi) efficiency. Unexpectedly, we found that GFP fluorescence was significantly brighter in Verson’s glands than in other tissues. Also, RNAi efficiency improved more in Verson’s glands than in other tissues. We took advantage of improved RNAi efficiency to explore the function of Verson’s glands. RNA-seq analysis revealed that genes highly expressed in Verson’s glands code for cuticular proteins, molting fluid proteins, hemolymph proteins, and antimicrobial peptides. Injection of dsRNA targeting essential genes interfered with Verson’s glands growth. These studies revealed that Verson’s glands contribute to hemolymph, cuticle, molting fluid, and immune response during molting. These studies also provide useful tools for future research in identifying the physiological role of Verson’s glands in lepidopteran insects.

2023-03-01 | GSE220983 | GEO

Fall armyworm and corn earworm gut microbiome

Project description:Fall armyworm and corn earworm gut microbiome Metagenome

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Fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) Whole Gut bacteria

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Multiple Evolutionary Pathways to Host Adaptation in the Fall Armyworm

Project description:During the over 300 million years of co-evolution between herbivorous insects and their host plants, a dynamic equilibrium of evolutionary arms race has been established. However, the co-adaptation between insects and their host plants is a complex process, often driven by multiple evolutionary mechanisms. We found that various lepidopteran pests that use maize as a host exhibit differential adaptation to the plant secondary metabolites, benzoxazinoids (BXs). Notably, the Spodoptera genus, including Spodoptera frugiperda (fall armyworm) and Spodoptera litura (cotton leafworm), demonstrate greater tolerance to BXs compared to other insects. Through comparative transcriptomic analysis of the midgut, we identified four candidate genes potentially involved in BXs detoxification in S. frugiperda. Subsequently, we confirmed two UGT genes, Sfru33T10 and Sfru33F32, as key players in BXs detoxification using CRISPR/Cas9 gene-editing technology. Phylogenetic analysis revealed that Sfru33T10 evolved independently within the Noctuidae family and is involved in the glycosylation of HDMBOA, while Sfru33F32 evolved independently within the Spodoptera genus and functions as a key detoxification enzyme responsible for the glycosylation of both DIMBOA and HMBOA. Our study demonstrates that the UGT gene family plays a crucial role in the adaptation of noctuid insects to maize, with multiple independent evolutionary events within the Noctuidae family and the Spodoptera genus contributing significantly to host adaptation.

2024-09-18 | GSE276946 | GEO

Multiple Evolutionary Pathways to Host Adaptation in the Fall Armyworm

Project description:Multiple Evolutionary Pathways to Host Adaptation in the Fall Armyworm

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Barcoding of Fall Armyworm

Project description:Fall armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) is an invasive and noxious pest of maize (Zea mays), which is native to tropical and subtropical regions of America. It is now an invasive pest of Indian Sub continent, first reported from Karnataka on maize in 2018.

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Differential transcriptome analysis reveals genes related to low- and high-temperature stress in the fall armyworm (Spodoptera frugiperda)

Project description:Differential transcriptome analysis reveals genes related to low- and high-temperature stress in the fall armyworm (Spodoptera frugiperda)

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Project description:Fall armyworm Gut Microbiota

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whale fall metagenome

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