Ontology highlight
ABSTRACT:
PROVIDER: PRJNA924540 | ENA |
REPOSITORIES: ENA
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Project description:Wheat (Triticum aestivum L.), one of the most important crops in the world, is a staple food used for making flour, noodles, alcoholic beverages, biofuel and a variety of other products. In wheat, kernel texture and biochemical composition vary with different hardnesses. Previous study of our research group indicated that physiological properties changes including germination ratio, and physiological enzymatic activities in wheat with different hardnesses were different. A comparative proteomic analysis of soft and hard wheat embryos by our research group identified more proteins associated with anti-resistance in soft wheat than that in hard wheat. Additionally, quantitative proteomic analysis of medium-hard wheat ‘Aikang58’ seeds showed that 162 differentially expressed proteins (DEPs) participated in metabolism, energy supply, and defense/stress responses were identified during artificial ageing were identified in our previous research. However, the dynamic physiological and quantitative proteomic changes in soft wheat seeds during accelerated aging remain unclear, thus need to be elucidated. In this work, we conducted the first Tandem Mass Tag (TMT)-based dynamic quantitative proteomic analysis of elite Chinese soft wheat cultivar ‘Yangmai 15’ seeds during artificial ageing. The uncovered differentially expressed proteins (DEPs) with different functions might provide new insights into the comprehensive understanding of deterioration in soft wheat seeds.
2018-10-08 | PXD009156 | Pride
Project description:We performed RNA-seq and Ribo-seq analyses to elucidate the translation in seeds at 85 and 115 DAF. We also completed a data-independent acquisition (DIA)-based proteomic analysis, while also examining relevant lipid metabolites.
2023-03-01 | PXD040512 |
Project description:Seeds germination is seriously sensitive to salt stress. The mechanism in response to salt stress during seed germination is still little known. In this study, two genotypes of hulless barley lk621 and lk53 were selected to investigate the molecular mechanism of seeds salinity response during germination stage through RNA-seq and iTRAQ technologies
2020-05-27 | PXD016100 | Pride
Project description:Seeds of Pisum sativum L. cv Alaska Early (purchased from the ‘Abundant Life Seed Foundation’, Port Townsend, USA) were equilibrated for approximately 5 weeks in tightly sealed boxes over 300 g-1 non-saturated LiCl (Hay et al. 2008), producing 60% relative humidity, in a temperature-controlled room (20 ± 1 ºC) until their water potential was stable at 12 % MC. Relative humidity was recorded with a Rotronic AWVC-D10 Hygropalm. These equilibrated seeds are referred to as 0 day ‘non-aged controls’. Following equilibration, accelerated ageing was carried out by placing seeds in sealed bottles at 50 °C until viability loss, thereafter referred to as ‘ageing’. Samples were taken at intervals after 8, 12, and 15 days of ageing. At each interval, germination tests were performed on 1 % water agar at 25 °C under warm white fluorescent light (15 micromol m-2 s-1) at a day / night cycle (8/16h). Germination was defined as radical emergence by at least 2 mm. To summarize, we have performed a transcriptional profiling of Pisum sativum seeds comparing 0 day ‘non-aged controls’ with 8, 12 and 15 days of artificially aged seeds.
2010-10-22 | GSE24864 | GEO
Project description:To provide a more detailed insight into molecular changes in seeds exposed to the accelerated ageing process, we excised embryos and compared the differences in the proteome composition. Seeds collected from four contrasting locations were exposed to accelerated ageing. Next, embryos were dissected and analyzed in five biological replicates [1-5]. Sample description:CAS [1-5] - location n. 5 - (Caslav) - high yield, above average temperature and high rainfall; a significant decrease in germination energy after the accelerated ageing test. UHO [1-5] - location n. 11 (Uhersky Ostroh) - low yield, high temperature and low rainfall; very low decrease in germination energy after the accelerated ageing test.CH [1-5] - location n. 2 (Chrastava) - average yield, low temperature, very high rainfall; a significant decrease in germination energy after the accelerated ageing test.K [1-5] - location n. 8 (Krasne Udoli) - average yield, low temperature, average rainfall; a significant decrease in germination energy after the accelerated ageing test.
2023-02-07 | PXD025075 | Pride
Project description:Panax notoginseng (Burk) F.H. Chen is an importantly economical and medicinal plant of the family Araliacease, and its seeds are obviously characterized by the recalcitrance and after-ripening process. an isobaric tag for relative and absolute quantification (iTRAQ) and RNA-seq was used to analyze the proteomic changes and transcriptomic in seeds of P. notoginseng during the after-ripening process .
2021-09-15 | PXD028471 |