Ontology highlight
ABSTRACT:
PROVIDER: PRJNA970194 | ENA |
REPOSITORIES: ENA
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Project description:Apple seeds were subjected to accelerated aging. After 7, 14, and 21 days of aging, embryos were isolated. Part of the embryos were shortly fumigated with nitric oxide (NO). After 48 h of embryos culture (aged embryos or aged embryos treated with NO), embryonic axes were used to extract the total RNA. RT-qPCR were done to analyze the changes in the expression of genes related to seed aging. Short-term (3 h) treatment of embryos isolated from accelerated aged apple seeds (Malus domestica Borkh.) with NO partially reduced the effects of aging. The aim of the study was to investigate the impact of the short-term NO treatment of embryos isolated from apple seeds subjected to accelerated aging on the expression of genes potentially linked to the regulation of seed aging. Apple seeds were artificially aged for 7, 14, or 21 days. Then the embryos were isolated from the seeds, treated with NO, and cultured for 48 h. Progression of seeds aging was associated with the decreased transcript levels of most of the analyzed genes (Lea1, Lea2a, Lea4, Hsp70b, Hsp20a, Hsp20b, ClpB1, ClpB4, Cpn60a, Cpn60b, Raptor, and Saur). The role of NO in the mitigation of seed aging depended on the duration of the aging. After 7 and 14 days of seed aging, a decreased expression of genes potentially associated with the promotion of aging (Tor, Raptor, Saur) was noted. NO-dependent regulation of seed aging was associated with the stimulation of the expression of genes encoding chaperones and proteins involved in the repair of damaged proteins. After NO application, the greatest upregulation of ClpB, Pimt was noted in the embryos isolated from seeds subjected to 7-day long accelerated aging, Hsp70b, Hsp70c, Cpn in the embryos of seeds aged for 14 days, and Lea2a in the embryos of seeds after 21 days of aging.
2022-07-07 | GSE207591 | GEO
Project description:We performed RNA-seq and Ribo-seq analyses to elucidate the translation in seeds at 85 and 115 DAF. We also completed a data-independent acquisition (DIA)-based proteomic analysis, while also examining relevant lipid metabolites.
2023-03-01 | PXD040512 |
Project description:Wheat (Triticum aestivum L.), one of the most important crops in the world, is a staple food used for making flour, noodles, alcoholic beverages, biofuel and a variety of other products. In wheat, kernel texture and biochemical composition vary with different hardnesses. Previous study of our research group indicated that physiological properties changes including germination ratio, and physiological enzymatic activities in wheat with different hardnesses were different. A comparative proteomic analysis of soft and hard wheat embryos by our research group identified more proteins associated with anti-resistance in soft wheat than that in hard wheat. Additionally, quantitative proteomic analysis of medium-hard wheat ‘Aikang58’ seeds showed that 162 differentially expressed proteins (DEPs) participated in metabolism, energy supply, and defense/stress responses were identified during artificial ageing were identified in our previous research. However, the dynamic physiological and quantitative proteomic changes in soft wheat seeds during accelerated aging remain unclear, thus need to be elucidated. In this work, we conducted the first Tandem Mass Tag (TMT)-based dynamic quantitative proteomic analysis of elite Chinese soft wheat cultivar ‘Yangmai 15’ seeds during artificial ageing. The uncovered differentially expressed proteins (DEPs) with different functions might provide new insights into the comprehensive understanding of deterioration in soft wheat seeds.
2018-10-08 | PXD009156 | Pride