Gallus gallus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA92751 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:A major research focus in our laboratory is the development of the chicken pituitary gland. As this tissue does not yield sufficient RNA to analyze using cDNA microarrays, mRNA must be amplified by reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter site followed by in vitro transcription using T7 RNA polymerase. Therefore, this preliminary study investigating differential gene expression between embryonic day 17, when all the major cell types in the chick anterior pituitary gland have differentiated, and post-hatch day 3 was conducted to verify that amplified antisense RNA could be used in conjunction with our neuroendocrine microarrays containing 5,128 unique cDNAs to detect changes in gene expression. Of the 662 cDNAs that were expressed at significantly different levels between the two ages (P < 0.05, n=3 samples/age), 159 exhibited substantial differences where the higher mean was at least 1.6-fold greater than the lower mean. The majority of the genes (117) increased between e17 and d3. Several genes which may play a role in neuroendocrine development were identified, including many transcription factors, signaling molecules, and morphogeneic factors. Keywords: time course during development (2 ages)
2005-09-02 | GSE3226 | GEO
Project description:The anterior pituitary is comprised of five major hormone-secreting cell types which differentiate during embryonic development in a temporally distinct manner. Microarrays containing 5,128 unique cDNAs expressed in the neuroendocrine system were produced and used to identify genes with potential involvement in onset of thyroid-stimulating hormone beta-subunit, growth hormone, and prolactin gene transcription during chick embryonic development. We identified 352 cDNAs that were differentially expressed (P < 0.05 and the highest mean at least 1.6-fold greater than the lowest mean) on embryonic day 10, 12, 14, or 17, the period of thyrotroph, somatotroph, and lactotroph differentiation in the chicken. Consistent with cellular ontogeny in the chick anterior pituitary gland, thyroid-stimulating hormone beta-subunit mRNA increased steadily between embryonic day 10 and embryonic day 17, growth hormone mRNA increased between embryonic day 12 and embryonic day 17, and PRL mRNA did not increase until embryonic day 17. Expression of 149 cDNAs increased in a manner similar to thyroid-stimulating hormone beta-subunit mRNA and 67 decreased between embryonic day 10 and embryonic day 17, and some of these are likely associated with thyrotroph differentiation. Similarly, expression of 74 and 66 genes changed in a manner over the 4 ages that is consistent with them having a potential role in induction of growth hormone and prolactin mRNA, respectively. Among these candidate genes are numerous transcription factors and signaling molecules that have not previously been implicated in pituitary development. Keywords: time course during development (4 ages)
2005-09-02 | GSE3227 | GEO
Project description:After elevated and reduced incubation temperature during embryonic days (ED) 7-10 and 10-13 changes of gene expression were determined at ED 10, ED 13, and post-hatch at day (D) 35
2016-12-31 | GSE76671 | GEO
Project description:After elevated and reduced incubation temperature during embryonic days (ED) 7-10 and 10-13 changes of gene expression were determined at ED 10, ED 13, and post-hatch at day (D) 35
2016-12-31 | GSE76670 | GEO
Project description:Characterization of Glucocorticoid-Induced Changes in Gene Expression in the Embryonic Pituitary Gland.
| PRJNA96703 | ENA
Project description:Transcriptome Analysis of post-hatch chicken hypothalamus under thermoneutral and heat stress conditions.
| PRJNA339587 | ENA
Project description:We identified the microglial population in the chick anterior hypothalamus (AH) and showed that early-life thermal stress (on day 3 post-hatch) influences the inflammatory process in the AH, altering microglial transcriptomic signature in response to LPS treatment (on day 10 post-hatch).
2021-12-21 | GSE189566 | GEO
Project description:Purpose:We have used RNA-seq to examine of differentially expressed miRNAs in chicken leg muscle of three different development stages (11 embryo ages, 16 embryo ages, and 1 day old post hatch chick).The aims of this study are characterization of miRNAs differentially expressed in different developmental stage of chicken embryo, using RNA sequence sample. Methods: On this study we used two embryonic stage and one post hatch chick leg muscle of Xinghua chicken breed. Total RNA from E11 day embryo, E16 day embryo and 1 day post hatch chick was isolated by TRIzol following the manufacturer’s protocol (Invitrogen, CA, USA). Each stages were designed two samples, and the total samples were six (three group × two sample/group) and RNA samples of six individuals were pooled with equal amounts, and then were subjected to Illumina deep sequencing. Results: After eliminating adaptor and low-quality reads, a total of 5,302,700, 6,556,747, 5,359,793, 4,213,112, 7,112,885 and 7,469,939 clean reads were obtained in group E11 (E11.1-E11.2), group E16 (E16.1-E16.2) and group P1 (P1.1–P1.2) libraries, respectively. The clean reads were aligned to the chicken genome databases, miRBase, Rfam, RepBase and mRNA. Conclusions:To assess miRNA expression during chicken embryo skeletal muscle development, we sequenced and analyzed leg muscle at 11 day embryo age, 16 day embryo age, and 1 days post hatch.
2017-06-22 | GSE100321 | GEO
Project description:Pre- and Post-hatch transcriptomic response of chicken Musculus gastrocnemius to transient variation of incubation temperature
| PRJNA308300 | ENA
Project description:We identified the microglial population in the chick anterior hypothalamus (AH) and showed that early-life thermal stress (on day 3 post-hatch) influences the inflammatory process in the AH. We described alterations in genome-wide CpG methylation profile of hypothalamic microglia between heat-conditioned and non-conditioned 10-day-old chicks prior LPS challenge. We suppose that changes in CpG DNA methylation might be involved in reprogramming microglial function.
2021-12-21 | GSE189564 | GEO