Project description:Transcriptomics (using DNA microarrays) was used to quantitatively compare the terminal ileum from conventional and germ-free mice (female and male; C57BL/10A and BALB/c strains).
Project description:A proteomic dataset comparing organ tissue differences between germ-free mice to conventional mice. Additional files include supplementary tables for reanalysis.
Project description:L cells are one of enteroendocrine cells. To understand the role of gut microbiota in its regulation, germ-free and conventional reporter mouse strain was used to sort Lpos and Lneg populations using FACS. The cell population were sorted from both ileum and colon and were subjected to transcriptional profiling.
Project description:The gut microbiota exerts profound influence on poultry immunity and metabolism through mechanisms that yet need to be elucidated. Here we used conventional and germ-free chickens to explore the influence of the gut microbiota on transcriptomic along the gut-lung axis in poultry. Our results demonstrated a differential regulation of genes associated with innate immunity and metabolism in the spleen of germ-free birds.
Project description:The gut microbiota exerts profound influence on poultry immunity and metabolism through mechanisms that yet need to be elucidated. Here we used conventional and germ-free chickens to explore the influence of the gut microbiota on transcriptomic along the gut-lung axis in poultry. Our results demonstrated a differential regulation of genes associated with innate immunity and metabolism in the lungs of germ-free birds.
Project description:The gut microbiota exerts profound influence on poultry immunity and metabolism through mechanisms that yet need to be elucidated. Here we used conventional and germ-free chickens to explore the influence of the gut microbiota on transcriptomic along the gut-lung axis in poultry. Our results demonstrated a differential regulation of genes associated with innate immunity and metabolism in the caeca of germ-free birds.
Project description:Dietary lipids and gut microbiota may both influence adipose tissue physiology. By feeding conventional and germ-free mice high fat diets with different lipid compositon we aimed to investigate how dietary lipids and the gut microbiota interact to influence inflammation and metabolism in epididymal adipiose tissue (EWAT)
Project description:The effects of maternal microbiota on the fetal development was investigated by comparing tissues of fetuses from germ-free (GF) and normal (SPF) murine dams using RNA-seq and non-targeted metabolomics (for metabolomics data, see: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-022-02457-6). For RNA-seq, two E18.5 fetuses were collected from 6 GF dams and 6 SPF dams, and transcriptomes analyzed by QuantSeq in whole intestine, brain and placenta.
Project description:Dietary lipids and gut microbiota may both influence adipose tissue physiology. By feeding conventional and germ-free mice high fat diets with different lipid compositon we aimed to investigate how dietary lipids and the gut microbiota interact to influence inflammation and metabolism in the liver
Project description:This study aimed to quantify and compare the mRNA abundance of all major XPGs in liver and intestine between conventional (CV) and germ-free (GF) mice using RNA-Seq. The CV RNA-Seq data were previously uploaded to GEO database by the same research group (GSE79848). All CV and GF mice were age-matched, and were analyzed under the same conditions (diet, water, bedding, and animal facility).