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L-Arginine enhances oral keratinocyte proliferation under high glucose conditions through upregulation of CYP1A1, SKP2, and SRSF5

ABSTRACT: L-Arginine enhances oral keratinocyte proliferation under high glucose conditions through upregulation of CYP1A1, SKP2, and SRSF5

PROVIDER: PRJNA928351 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23241521_1.fastq.gz	Fastqsanger.gz
	SRR23241521_2.fastq.gz	Fastqsanger.gz
	SRR23241522_1.fastq.gz	Fastqsanger.gz
	SRR23241522_2.fastq.gz	Fastqsanger.gz
	SRR23241523_1.fastq.gz	Fastqsanger.gz

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L-Arginine enhances oral keratinocyte proliferation under high glucose conditions through upregulation of CYP1A1, SKP2, and SRSF5

Project description:L-Arginine promotes oral keratinocyte proliferation under high glucose conditions. The treatment leads to changes in a substantial number of gene transcripts related to cell cycle, cell proliferation, and spliceosome GO terms as well as signaling pathways. Among these top-upregulated genes, CYP1A1, SKP2, and SRSF5 are of particular significance.

2023-10-10 | GSE223773 | GEO

L-Arginine Enhances Oral Keratinocyte Proliferation under High-Glucose Conditions via Upregulation of CYP1A1, SKP2, and SRSF5.

Project description:High glucose inhibits oral keratinocyte proliferation. Diabetes can lead to delayed oral wound healing and periodontal disease. L-Arginine, one of the most versatile amino acids, plays an important role in wound healing, organ maturation, and development. In this study, L-Arginine was found to enhance oral keratinocyte proliferation under high-glucose conditions. RNA sequencing analysis discovered a significant number of genes differentially upregulated following L-Arginine treatment under high-glucose conditions. Cytochrome P450 family 1 subfamily A member 1 (CYP1A1) was the most significantly upregulated gene at 24 and 48 h after L-Arginine treatment. Gene Ontology enrichment analysis found that cell proliferation- and mitosis-related biological processes, such as mitotic nuclear division, mRNA processing, and positive regulation of cell cycle processes, were significantly upregulated. Pathway enrichment analysis found that S-phase kinase-associated protein 2 (SKP2) and serine- and arginine-rich splicing factor 5 (SRSF5) were the top upregulated genes in cell cycle and spliceosome pathways, respectively. Indirect immunofluorescent cytochemistry confirmed increased protein levels of CYP1A1, SKP2, and SRSF5 after L-Arginine treatment. Knockdown of CYP1A1, SKP2, and SRSF5 abolished the enhanced proliferative effect of L-Arginine on oral keratinocytes under high-glucose conditions. In conclusion, L-Arginine enhances oral keratinocyte proliferation under high-glucose conditions via upregulation of CYP1A1, SKP2, and SRSF5, suggesting that supplemental L-Arginine in oral care products may be beneficial for oral tissue repair and regeneration.

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