Project description:Gene expression microarray data in resting myeloid cells and CD4+ T cells, as well as in response to lipopolysaccharide (LPS) or phytohemagglutinin (PHA) stimulation for 24 hours, respectively. Myeloid cells and CD4+ T cells were purified from resting and stimulated peripheral blood mononuclear cells from cord blood samples of 152 individuals at birth. Raw array data and quantile normalised gene expression data are available. Full summary statistics of eQTL analysis and interaction tests for response eQTLs are available. Information such as sample attributes and experimental variables are available in the \\"Microarray_sample_info.txt\\".
Project description:To investigate novel molecular signatures and transcriptional regulators of immature and mature human NK cells, we performed whole-genome microarray analysis on dNK cells (CD3−CD56+), cNK cells (CD3−CD56+), pNK cells (CD3−CD56+), CD56+ T cells (CD3+CD56+) and T cells (CD3+CD56−). dNK cells were purified from first-trimester deciduas. cNK cells were purified from cord-blood mononuclear cells. pNK, CD56+ T and T cells were purified from adult peripheral blood mononuclear cells. Samples were collected from healthy adult donors after obtaining informed consent according to the Ethics Committee of the University of Science & Technology of China.
Project description:We performed single cell RNA sequencing to ensure that the engrafted MF cells in NSGS mice retained the molecular properties of the patients cells. ScRNAseq profiles from peripheral blood mononuclear cells (PBMCs) from two independent cord blood and MF patient samples were compared to the engrafted hCD45+ cells from the bone marrow of NSGS mice at 12-weeks post-transplant.
Project description:We isolated resting platelets from full term umbilical cord blood and adult peripheral blood. We used DIA mass spectrometry to analyze the whole proteome and phosphoproteome from this platelet samples.
Project description:Whole blood was collected from healthy adult and peripheral blood mononuclear cells (PBMC) were isolated. PBMCs were cultured in medium or stimulated with RNase treated EC-12 or untreated EC-12 for 6hours. Then transcriptome profiles in PBMC were obtained.
Project description:The purpose of the experiment was to transcriptionally characterize transgenic T cells derived from cord blood (CB) and cultured with IL-2 or IL-7 and IL-15, or from peripheral blood (PB) and cultured with IL-2. The sample replicates were generated using peripheral and cord blood mononuclear cells, obtained from healthy human donors and cord blood units that were not compliant with banking standards, respectively. T cells were then transduced with a retroviral vector encoding an HLA-DRB1*04-restricted TCR targeting the human papillomavirus oncoprotein E7.
