Project description:In order to test the hypothesis that fibroblasts from different tissues are phenotypically distinct from one another, we have subjected tendon, skin and corneal fibroblasts of fetal mouse to mechanical stimulation by fluid flow and analyzed the transcriptional responses of the cells using Affymetrix MOE430 chip set containing two arrays MOE430A and MOE430B. Experiment Overall Design: The experiment is done using Affymetrix MOE430 chip set containing two arrays MOE430A - platform GPL339 and MOE430B - platform MOE340. Three biological replicates for tendon, skin and corneal fibroblasts subjected to mechanical stimulation as well as not stimulated (control) were analyzed yielding 18 samples per chip (36 in total).
Project description:In order to test the hypothesis that fibroblasts from different tissues are phenotypically distinct from one another, we have subjected tendon, skin and corneal fibroblasts of fetal mouse to mechanical stimulation by fluid flow and analyzed the transcriptional responses of the cells using Affymetrix MOE430 chip set containing two arrays MOE430A and MOE430B. Keywords: cell type comparison, stress response
Project description:We used microRNA microarrays (Affy miRNA 2.0 array) to compare the miRNA expression between proliferating (mock) and different time points (12, 48, 72 hours) of serum starvation (to induce quiescence) in Human Foreskin Fibroblast (HFF) cells.
Project description:In this study, using chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq), we identified the main binding sites of catalytically inactive Cas9 (dCas9) protein in bovine fetal fibroblast cells (BFFs) in an unbiased manner.
Project description:Oral squamous cell carcinoma (OSCC) accounts for the high mortality rate and morbidity in head and neck cancer. Data for analysis were obtained from the microarray data of 6 OSCC tissues, tissues adjacent to cancer, and contralateral normal tissues.We used the affy package under the R environment to preprocess and normalize the original gene chip data.
Project description:Oral squamous cell carcinoma (OSCC) accounts for the high mortality rate and morbidity in head and neck cancer. Data for analysis were obtained from the microarray data of 6 OSCC tissues, tissues adjacent to cancer, and contralateral normal tissues.We used the affy package under the R environment to preprocess and normalize the original gene chip data.
Project description:The production of skeletal muscle constructs useful for in vivo large defects replacement, such as congenital diaphragmatic hernia (CDH), is still considered a challenge. The standard application of prosthetic material presents strong limitations, like hernia recurrences in a remarkable number of CDH patients. With this work, we developed a tissue engineering approach based on decellularized diaphragmatic muscle and human cells for the generation in vitro of diaphragmatic-like tissues as proof-of-concept of a new option for the surgical treatment of large diaphragm defects. A specific bioreactor for diaphragmatic muscle was achieved to control mechanical stimulation during the construct generation. In vitro tests demonstrated the increased maturation of mechanically stimulated constructs, with the formation of new oriented and aligned muscle fibres. Moreover, after in vivo orthotopic implantation in a CDH mouse model, mechanically stimulated muscles maintained the presence of human cells within myofibers, supporting the idea of a new therapeutic option for this dramatic congenital malformation. Production of diaphragmatic-like tissues using mouse decellularized extracellular matrix, human cells and mechanical stimulation with bioreactor
Project description:Using two complementary approaches, analysis of imprinting of candidate genes by pyrosequencing and expression profiling of parthenogenetic fetuses, we carried a comprehensive survey of genomic imprinting in swine. In the case of imprinted genes where transcription of one of the two parental alleles is silenced, uniparental embryos like parthenotes can be used to measure transcript dosage effects. Using Affymetrix Porcine GeneChip microarrays, four tissues of day 30 fetuses were profiled: brain, fibroblast, liver, and placenta. Keywords: Transcriptional profiling of epigenetic asymmetry in porcine day 30 parthenogenetic and biparental fetal tissues 24 samples total of Day 30 occidental porcine fetuses: 2 treatments (parthenote, control); four tissues (brain, fibroblast, liver, placenta); technical replicates (3 fibroblast only)
Project description:We undertook gene expression microarray experiments to identify genes that are differentially expressed in different placental and fetal tissue, and resting and Pokeweed Mitogen (PWM) stimulated horse lymphocytes. Conceptus tissues were dissected to obtain chorionic girdle, chorion, and fetal tissue. Freshly isolated horse peripheral blood lymphocytes were split and harvested immediately, or stimulated with PWM and harvested over a five day period. These experiments utilized a custom Agilent horse array designed in house that featured >14,000 probes on an 8x15k array format. Several genes were selected from the results for validation by quantitative real-time PCR. QPCR results matched the microarray results very closely.
Project description:this data set includes deep targeted re-sequencing of fetal bulk tissues of the 4 foetuses (T21=2, D21=2). The tissues include: fetal skin and intestinal organoid cultures passage 0 of all 4 fetuses, and spleen of fetus N01 (T21)