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B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection

ABSTRACT: B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection

PROVIDER: PRJNA936432 | ENA |

REPOSITORIES: ENA

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B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection [bulk RNA-seq]

Project description:B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection [bulk RNA-seq]

| PRJNA936434 | ENA

B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection [single cell RNA-Seq]

Project description:B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection [single cell RNA-Seq]

| PRJNA936433 | ENA

B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection [bulk RNA-seq]

Project description:Germinal centers (GCs) are sites of B cell clonal expansion, diversification, and antibody affinity selection. This process is limited and directed by T follicular helper cells that provide helper signals to B cells that endocytose, process, and present cognate antigens in proportion to their B cell receptor (BCR) affinity. Under this model, the BCR functions as an endocytic receptor for antigen capture. How signaling through the BCR contributes to selection is not well understood. To investigate the role of BCR signaling in GC selection, we developed a tracker for antigen binding and presentation and a Bruton’s tyrosine kinase drug-resistant-mutant mouse model. We showed that BCR signaling per se is necessary for the survival and priming of light zone B cells to receive T cell help. Our findings provide insight into how high-affinity antibodies are selected within GCs and are fundamental to our understanding of adaptive immunity and vaccine development.

2023-03-09 | GSE225573 | GEO

B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection [single cell RNA-Seq]

2023-03-09 | GSE225574 | GEO

B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection.

Project description:Germinal centers (GCs) are sites of B cell clonal expansion, diversification, and antibody affinity selection. This process is limited and directed by T follicular helper cells that provide helper signals to B cells that endocytose, process, and present cognate antigens in proportion to their B cell receptor (BCR) affinity. Under this model, the BCR functions as an endocytic receptor for antigen capture. How signaling through the BCR contributes to selection is not well understood. To investigate the role of BCR signaling in GC selection, we developed a tracker for antigen binding and presentation and a Bruton's tyrosine kinase drug-resistant-mutant mouse model. We showed that BCR signaling per se is necessary for the survival and priming of light zone B cells to receive T cell help. Our findings provide insight into how high-affinity antibodies are selected within GCs and are fundamental to our understanding of adaptive immunity and vaccine development.

| S-EPMC10424567 | biostudies-literature

B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-08 | GSE225575 | GEO

Myc transcriptional bursts promote B cell positive selection by T cells in germinal centers

Project description:Antibody affinity maturation occurs in secondary lymphoid organs within germinal centers (GCs) where B cells mutate their antibody-encoding genes in the dark zone (DZ) followed by a selection of the high-affinity variants in the light zone (LZ) by T cells. The amount of the T cell-derived selection signals is proportional to the BCR affinity and the magnitude of Myc expression. However, since the lifetimes of Myc mRNA and protein are extremely short, it is unclear how high levels of Myc are maintained in the GC B cell to allow positive selection. Here, by measuring Myc transcripts in situ at the single-cell level, we find that T cell help promotes the frequency of Myc transcriptional bursts. T cell help primarily increases the number of Myc-positive cells rather than the amount of Myc per cell. Measurement of Myc intronic and exonic transcripts in situ demonstrates that positive selection is mediated by Myc transcriptional bursts through a rapid increase in the active transcription sites rather than an elevation in transcription rate or gene accessibility. Thus, the amount of Myc transcriptional bursts over time in the LZ, rather than Myc abundance, dictates B cell selection in germinal centers.

2024-10-15 | GSE237128 | GEO

Peyer’s Patch Germinal Centers Select Innate-like BCRs

Project description:Peyer’s Patch Germinal Centers Select Innate-like BCRs

| PRJNA591005 | ENA

Proteome profiling of mouse germinal center B cells

Project description:Productive B cell responses are critical to protect a host from infection. The spleen and lymph nodes are populated by resting follicular B cells, which can enter germinal centers upon antigen encounter. Once in the germinal center, B cells migrate between the dark and light zones, where they undergo somatic hypermutation and selection, respectively. While germinal center B cells have been studied, an intense molecular understanding of these cells/subsets (and the differences between them) is lacking.

2020-04-06 | PXD015524 | Pride

Myc transcriptional bursts promote B cell positive selection by T cells in germinal centers

Project description:Myc transcriptional bursts promote B cell positive selection by T cells in germinal centers

| PRJNA994027 | ENA