Project description:The thermotolerant TTY23, acid tolerant AT22, and thermo-acid tolerant TAT12 yeast strains, generated by adaptive evolution experiments were grown at 30°C, pH 5.0 in the absence of acetic acid (optimal ancestral conditions). The mRNA of each strain was isolated and changes in expression were compared against the parental strain S288C.
Project description:Saccharomyces cerevisiae reference strain S288C was used to obtain petites. FIP is fluconazole induced petite. EIP is EtBr induced petite. S288C, FIP and EIP were grown in YPD broth from OD600=0.1 till 1.0. Cells were collected and transcriptomes were analyzed.
Project description:S. cerevisiae strain S288C was subjected to CRISPR/Cas9 edition in the HMG-CoA reducatase gene using a modified housekeeping promoter ADH1 incorporating the up-stream region of TEF1 promoter (UADH1). Mutant strain was called S288C-H2.
Project description:S288C was transformed with plasmids expressing the GCN5 F221A mutant at varying levels. We sought to examine the global impact on gene expression using the Affymetrix yeast 2.0 arrays.