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ABSTRACT: study on the function of NorR regulator in Vibrio

PROVIDER: PRJNA945216 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23874500_1.fastq.gz	Fastqsanger.gz
	SRR23874500_2.fastq.gz	Fastqsanger.gz
	SRR23874494_1.fastq.gz	Fastqsanger.gz
	SRR23874494_2.fastq.gz	Fastqsanger.gz
	SRR23874495_1.fastq.gz	Fastqsanger.gz

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Dual RNA regulator VcdRP in V. cholerae modulates central metabolism

Project description:Bacterial small RNAs (sRNAs) are well-known to modulate gene expression by base-pairing with trans-coded transcripts and are typically considered to be non-coding. However, several sRNAs have been reported to also contain an open reading frame and thus are considered dual-function regulators. We discovered a dual-function regulator from Vibrio cholerae, called VcdRP, harboring a 29 amino acid protein (VcdP), as well as a base-pairing sequence. In this study, we measured the metabolite abundance of glycolytic and citric acid cycle intermediates using LC-MS.

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Project description:In this study we determined the target spectrum of the Vibrio cholerae dual RNA regulator VcdRP via pulse-expression of different variants followed by RNA-sequencing.

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ChIP-seq identified the regulation of genes by the quorum-sensing regulator OpaR in Vibrio parahaemolyticus

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Project description:In marine Vibrio species, chitin-induced natural transformation enables bacteria to take up DNA from the external environment and integrate it into their genome via homologous recombination. Expression of the master competence regulator TfoX bypasses the need for chitin induction and drives expression of the genes required for competence in several Vibrio species. Here, we show that TfoX expression in two Vibrio campbellii strains, DS40M4 and NBRC 15631, enables high frequencies of natural transformation. Conversely, transformation was not achieved in the model quorum-sensing strain V. campbellii BB120 (previously classified as Vibrio harveyi). Surprisingly, we find that quorum sensing is not required for transformation in V. campbellii DS40M4. This result is in contrast to Vibrio cholerae that requires the quorum-sensing regulator HapR to activate the competence regulator QstR. However, similar to V. cholerae, QstR is necessary for transformation in DS40M4. To investigate the difference in transformation frequencies between BB120 and DS40M4, we used previously studied V. cholerae competence genes to inform a comparative genomics analysis coupled with transcriptomics. BB120 encodes homologs of all known competence genes, but most of these genes were not induced by ectopic expression of TfoX, which likely accounts for the non-functional natural transformation in this strain. Comparison of transformation frequencies among Vibrio species indicates a wide disparity among even closely related strains, with Vibrio vulnificus having the lowest functional transformation frequency. We show that ectopic expression of both TfoX and QstR is sufficient to produce a significant increase in transformation frequency in Vibrio vulnificus.

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Project description:Study of the effect of L-Arabinose on chromosomes replication forks progression in Vibrio cholerae. Processed wig.mat file.

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