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Transcriptome analysis upon lymphatic endothelial cells (LEC) and cancer cells (Haca4) co-culture

ABSTRACT: Transcriptome analysis upon lymphatic endothelial cells (LEC) and cancer cells (Haca4) co-culture

PROVIDER: PRJNA946667 | ENA |

REPOSITORIES: ENA

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Transcriptome analysis upon lymphatic endothelial cells (LEC) and cancer cells (Haca4) co-culture

Project description:To investigate the mechanisms underlying the connection between LEC and cancer cells and their potential roles in tumorigenesis we co-cultured LEC and HaCa4 cells for 24 hours and compared the transcript levels of both FACS-isolated HaCa4 cells and LEC from co-cultures with those from individual cell cultures

2023-04-21 | GSE227697 | GEO

Lymphatic endothelial cells (LEC) treated with a MAF-targeted siRNA

Project description:Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognisable by its distinctive red skin lesions. The lesions are KSHV-infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. The KSHV virus expresses multiple MAF-downregulating microRNA. Here we test the effects of MAF silencing by siRNA in LEC cells using Affymetrix hgu133plus2 chips.

2009-09-30 | GSE16356 | GEO

Lymphatic endothelial cells (LEC) transfected with the KSHV microRNA cluster

Project description:Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognisable by its distinctive red skin lesions. The lesions are KSHV-infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. The KSHV virus expresses multiple microRNA in a single cluster. Here we test the effects of this KSHV microRNA cluster in LEC cells using Affymetrix hgu133plus2 chips.

2009-09-30 | GSE16355 | GEO

Lymphatic endothelial cells comparison gene array data

Project description:GeneChipÂ® Mouse Gene 2.0 ST Array for C57BL/6 mouse skin dermal primary lymphatic endothelial cells (Ms LEC) and mouse lymphatic endothelial cell line SVEC4-10 GeneChipÂ® Human Gene 2.0 ST Array for human primary lymphatic endothelial cells (Hu LEC) Total RNA from lymphatic cell line SVEC4-10 were used for GeneChipÂ® Mouse Gene 2.0 ST Array. SVEC4-10 samples, human and mouse LEC samples.

2016-07-20 | E-GEOD-84551 | biostudies-arrayexpress

Infection of Lymphatic and Blood Vessel Endothelial Cells (LEC and BEC) with KSHV

Project description:Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognisable by its distinctive red skin lesions. The lesions are KSHV-infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. The effects of KSHV infection of both LEC and BEC were assayed using Affymetrix hgu133plus2 chips at 72 hours post infection.

2009-09-30 | GSE16354 | GEO

Expression profiling of tumor lymphatic endothelial cells upon acute Ang2 inhibition

Project description:Lymphatic endothelial cells (LEC) were isolated from MT-ret tumor tissue 12h after treatment with an Ang2-blocking antibody or control-IgG

2020-12-01 | GSE141262 | GEO

Lymphatic endothelial cells comparison gene array data

Project description:GeneChip® Mouse Gene 2.0 ST Array for C57BL/6 mouse skin dermal primary lymphatic endothelial cells (Ms LEC) and mouse lymphatic endothelial cell line SVEC4-10 GeneChip® Human Gene 2.0 ST Array for human primary lymphatic endothelial cells (Hu LEC) Total RNA from lymphatic cell line SVEC4-10 were used for GeneChip® Mouse Gene 2.0 ST Array.

2016-07-20 | GSE84551 | GEO

MicroRNA expression in human lymphatic endothelial cells (LEC) and blood endothelial cells (BEC)

Project description:MicroRNA expression in human lymphatic endothelial cells (LEC) and blood endothelial cells (BEC)

| PRJNA522973 | ENA

Transcription profiling of lymphatic endothelial cells infected with Kaposi's sacoma associated herpes virus

Project description:This SuperSeries is composed of the following subset Series: GSE16353: The profile of cellular and KSHV microRNAs in AIDS_KS biopsies (and normal skin control biopsies) GSE16354: Infection of Lymphatic and Blood Vessel Endothelial Cells (LEC and BEC) with KSHV GSE16355: Lymphatic endothelial cells (LEC) transfected with the KSHV microRNA cluster GSE16356: Lymphatic endothelial cells (LEC) treated with a MAF-targeted siRNA Refer to individual Series

2009-09-30 | E-GEOD-16357 | biostudies-arrayexpress

Metabolomics revealed the influence of breast cancer on lymphatic endothelial cell metabolism, metabolic crosstalk, and lymphangiogenic signaling in co-culture

Project description:<p>Breast cancer metastasis occurs via blood and lymphatic vessels. Breast cancer cells 'educate' lymphatic endothelial cells (LECs) to support tumor vascularization and growth. However, despite known metabolic alterations in breast cancer, it remains unclear how lymphatic endothelial cell metabolism is altered in the tumor microenvironment and its effect in lymphangiogenic signaling in LECs. We analyzed metabolites inside LECs in co-culture with MCF-7, MDA-MB-231, and SK-BR-3 breast cancer cell lines using 1H nuclear magnetic resonance (NMR) metabolomics, Seahorse, and the spatial distribution of metabolic co-enzymes using optical redox ratio imaging to describe breast cancer-LEC metabolic crosstalk. LECs co-cultured with breast cancer cells exhibited cell-line dependent altered metabolic profiles, including significant changes in lactate concentration in breast cancer co-culture. Cell metabolic phenotype analysis using Seahorse showed LECs in co-culture exhibited reduced mitochondrial respiration, increased reliance on glycolysis and reduced metabolic flexibility. Optical redox ratio measurements revealed reduced NAD(P)H levels in LECs potentially due to increased NAD(P)H utilization to maintain redox homeostasis. 13C-labeled glucose experiments did not reveal lactate shuttling into LECs from breast cancer cells, yet showed other 13C signals in LECs suggesting internalized metabolites and metabolic exchange between the two cell types. We also determined that breast cancer co-culture stimulated lymphangiogenic signaling in LECs, yet activation was not stimulated by lactate alone. Increased lymphangiogenic signaling suggests paracrine signaling between LECs and breast cancer cells which could have a pro-metastatic role.</p>

2024-06-25 | MTBLS859 | MetaboLights

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