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Characterizing adjuvants’ effects at the murine immunoglobulin repertoire level

ABSTRACT: Characterizing adjuvants’ effects at the murine immunoglobulin repertoire level

PROVIDER: PRJNA951738 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24055300_1.fastq.gz	Fastqsanger.gz
	SRR24055300_2.fastq.gz	Fastqsanger.gz
	SRR24055301_1.fastq.gz	Fastqsanger.gz
	SRR24055301_2.fastq.gz	Fastqsanger.gz
	SRR24055302_1.fastq.gz	Fastqsanger.gz

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Characterizing adjuvants’ effects at the murine immunoglobulin repertoire level

Project description:We developed an improved library prep protocol and standardized the data analysis pipeline for accurate repertoire profiling. In addition, two metrics were implemented to assess repertoire clone properties. We then studied systemically the effects of two adjuvants, CpG and Alum, on the Ig heavy chain repertoire using the ovalbumin (OVA) challenged mouse model. Ig repertoires of different tissues (spleen and bone marrow) and isotypes (IgG and IgM) were examined and compared in terms of sequence mutation frequency, IGHV gene usage, CDR3 length, rescaled Hill numbers for clonal diversity, and clone selection strength. As a result, Ig repertoires of different tissues or isotypes exhibited distinguishable profiles at the non-immunized steady state. Adjuvanted immunizations further resulted in statistically significant alterations in Ig repertoire compared with PBS or OVA alone immunized groups. Lastly, we applied unsupervised machine learning techniques – multiple factor analysis and clustering – to identify Ig repertoire signatures in different compartments and under varying immunizations.

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