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BACH1 deficiency impedes differentiation of vascular smooth muscle cell from hESCs via reducing the CARM1-catalyzed H3R17me2

ABSTRACT: BACH1 deficiency impedes differentiation of vascular smooth muscle cell from hESCs via reducing the CARM1-catalyzed H3R17me2

PROVIDER: PRJNA954047 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24121624_1.fastq.gz	Fastqsanger.gz
	SRR24121624_2.fastq.gz	Fastqsanger.gz
	SRR24121625_1.fastq.gz	Fastqsanger.gz
	SRR24121625_2.fastq.gz	Fastqsanger.gz
	SRR24121626_1.fastq.gz	Fastqsanger.gz

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BACH1 deficiency impedes differentiation of vascular smooth muscle cell from hESCs via reducing the CARM1-catalyzed H3R17me2

Project description:Vascular smooth muscle cells (VSMCs) derived from human embryonic stem cells (hESCs) have been considered as a potential therapeutic application in vascular diseases. The transcription factor BTB and CNC homology 1 (BACH1) participates in stem cell development and has an increased expression during the VSMCs differentiation process. Knockout of BACH1 inhibits the differentiation of hESCs into VSMCs, whereas overexpression of BACH1 promotes VSMCs differentiation after the mesoderm stage. Mechanistically, BACH1 interacts with Coactivator-associated arginine methyltransferase 1 (CARM1) in a bZIP domain-dependent manner. BACH1 recruits CARM1 and its specific histone mark H3R17me2 to VSMC marker genes promoters, thus up-regulating target gene expression. BACH1-induced promotion of VSMC marker genes was partially abolished by the knockdown of CARM1 or H3R17me2. Thus, our results demonstrate the regulatory role of BACH1 and CARM1 in VSMCs differentiation.

2023-11-17 | GSE229351 | GEO

CARM1 substrate identification

Project description:The goal of the project was global identification of CARM1 substrates. Arginine methylation landscapes were profiled and compared in wild type and CARM1 knockout cells to determine in vivo substrates of CARM1.

2017-07-03 | PXD005871 | Pride

Gene expression profiles of CARM1 overexpression in MCF7

Project description:The goal of this study is to identify ERalpha-target genes affected by overexpression of the histone arginine methyltransferase CARM1 in breast cancer cells. The roles of CARM1 in ERalpha+ breast cancer was not well characterized. Therefore, we created a Dox inducible CARM1 overexpressing MCF7 cell line where CARM1 is overexpressed by 2 fold to determine the created a Dox-inducible CARM1 overexpressing MCF7 cells for evaluation of the global effects of CARM1 on Eralpha-target gene expression. MCF7-tet-on-CARM1 clone 13 were treated under 4 conditions: DMSO; Dox; E2 (10nM); Dox+E2. In Dox+E2 condition, cells were pre-treated with Dox for 5 days before treating with E2 for 4 hours. 3 biological replicates were included and total of 12 samples were analyzed.

2011-06-01 | E-GEOD-26454 | biostudies-arrayexpress

Genome-wide map of BACH1 binding in HEK 293T cells

Project description:BTB and CNC homology 1 (BACH1) is a heme-binding transcription factor repressing the transcription from a subset of MAF recognition elements (MAREs) at low intracellular heme levels. Upon heme binding, BACH1 is released from the MAREs, resulting in increased expression of antioxidant response genes. To systematically address the gene regulatory networks involving BACH1, we combined chromatin immunoprecipitation-sequencing (ChIP-seq) analysis of BACH1 target genes in HEK 293 cells with knock-down of BACH1 using three independent types of small interfering RNAs followed by transcriptome profiling using microarrays. The 59 BACH1 target genes identified by ChIP-seq were found highly enriched in genes showing expression changes after BACH1 knock-down, demonstrating the impact of BACH1 repression on transcription. In addition to known and new BACH1 targets involved in heme degradation (HMOX1, FTL, FTH1, ME1, SLC48A1) and redox regulation (GCLC, GCLM, SLC7A11), we also discovered BACH1 target genes effecting cell cycle and apoptosis pathways (ITPR2, CALM1, SQSTM1, TFE3, EWSR1, CDK6, BCL2L11, MAFG) as well as subcellular transport processes (CLSTN1, PSAP, MAPT, vault RNA). The newly identified impact of BACH1 on genes involved in neurodegenerative processes and proliferation provides an interesting basis for future dissection of BACH1-mediated gene repression in neurodegeneration and virus-induced cancerogenesis. Examination of BACH1 binding in HEK 293T cells by chromatin immunoprecipitation-sequencing (CHIP-seq) with input DNA as control.

2011-05-13 | E-GEOD-28051 | biostudies-arrayexpress

Role of BACH1 in HEK 293T cells

Project description:This SuperSeries is composed of the following subset Series: GSE28050: Expression data from knockdown of BACH1 in HEK 293T cells GSE28051: Genome-wide map of BACH1 binding in HEK293T cells Refer to individual Series

2011-05-12 | E-GEOD-28053 | biostudies-arrayexpress

Gene expression profiles of MCF7 with CARM1 knocked down

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2011-06-01 | E-GEOD-26259 | biostudies-arrayexpress

Global tibialis anterior skeletal muscle transcript data from male Carm1 skm-/- (HSAcre Tg/0 x Floxed Carm1) and Carm1 skm+/+ mice (HSAcre 0/0 x Floxed Carm1) on a chow diet.

Project description:Transcript data from tibialis anterior muscle from male muscle-specific Coactivator-Associated Arginine Methyltransferase 1 (Carm1 ) knockout mice (Carm1 skm-/- ) and wild-type mice (Carm1 skm+/+) generated by using the Cre-loxP system (on a C57BL6J/129 background) with HSA-Cre mice (from Jackson laboratories). We used RNA-Seq to detail the effects of muscle-specific loss of Carm1 on the global program of gene expression in tibialis anterior muscle.

2022-08-24 | GSE189351 | GEO

Regulation of immunity by Bach1 in Mice

Project description:Genes regulated by Bach1 transcription factor

2012-07-09 | E-MEXP-3674 | biostudies-arrayexpress

Dual BACH1 regulation by complementary SCF-type E3 ligases

Project description:BACH1, FBXO22, FBXL17, oxidative stress response, E3, Cullin-RING ligases, F-box, cysteine modification, S-nitrosylation, heme

2024-12-03 | PXD051074 | Pride

Transcription profiling of mouse embryonic fibroblasts carm1-/- completemented with CARM1 wt were left untreated or were stimulated for 3hours with 10ng/ml mTNFa

Project description:Complemented carm1-/- MEFs with CARM1 wt were left untreated or were stimulated for 3hours with 10ng/ml mTNFa. mTNFa-induced NF-kB dependent gene expression was analyzed on a custom array for specific NF-kB target genes

2009-09-01 | E-MEXP-2020 | biostudies-arrayexpress

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