Project description:Comparison of gene expression profiles of mouse bone marrow nucleated cells. All comparisons are made between biological replicates (different mice) derived from animals carrying the Type A cytoplasmic Nucleophosmin mutation (Npm1c) found in human Acute Myeloid Leukaemia. Mice were culled 8 weeks after induction of the conditional Npm1c allele with Mx1-Cre. Whole bone marrow (BM, n=20: 11xNpm1c & 9xWildType) or antibody purified cell fractions (Lin minus, n=6: 4xNpm1c & 2xWildType; B220+, n=5: 3xNpm1c & 2xWildType and Gr1+/Mac1+, n=6: 4xNpm1c & 2xWildType) were studied. Files ("samples") include data from all samples in each comparison.<br>Global profiling was done using Illumina mouse-6 expression beadchip version 2. Data were quantile normalised and analysed using the bioconductor (http://www.bioconductor.org/), lumi (http://www.bioconductor.org/packages/2.0/bioc/html/lumi.html ) and limma packages, then p-value adjusted for multiple testing.
Project description:Total RNA from abdominal walls of E10.5 mouse embryos that were wild type, heterozygote, or mutant at the Pitx2 locus were compared.. Three biological replicates were tested for each of the three genotypes.
Project description:We observed extensive neurite formation in NG108-15 cells cultured in the presence of the flavonoid, isoquercitrin. To help determine the mechanism of neuritogenesis, microarray analysis was performed on samples treated with 40 uM isoquercitrin for 24 hrs.
Project description:Exposing freshly isolated human articular chondrocytes to hyperosmotic conditions (550 mOsm vs 380mOsm controls) for 5 hours and then performing transcriptome analysis using Illumina Ref8 arrays.
Project description:Transcription profiling of FOXK2 expression in HeLa cells from a stably integrated doxycyline-inducible EGFP-FOXK2 expression construct.
Project description:Adipose tissue from mesenteric and subcutaneous depots from 11beta-HSD1 knockout (KO) and wild-type (WY) mice. Five biological replicates in each group: mesenteric KO, mesenteric WT, subcutaneous KO, subcutaneous WT.