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Gene Expression Changes in Soleus Muscle in Mice after Pericytes-Deletion

ABSTRACT: Gene Expression Changes in Soleus Muscle in Mice after Pericytes-Deletion

PROVIDER: PRJNA954284 | ENA |

REPOSITORIES: ENA

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Gene Expression Changes in Soleus Muscle in Mice after Pericytes-Deletion

Project description:Pericytes(PCs) are mural cells embedded in the capillary basal lamina. Some PC populations exhibit multipotency, similar to mesenchymal stem cells. Here, the the process of adaptation was investigated by determining changes in transcript profiles when NG2 positive PCs were deleted. PC-deletion was induced by using NG2CreER(+/-)/diphtheria toxin flagment A(DTA) transgenic mice. Cre recombinase was induced by tamoxifen(Tam) treatment(intraperitoneally, 100mg/kg body weight per day) for 5days. Samples were collected at 1month after Tam treatment.

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Project description:We used phosphoproteomic profiling of slow-twitch (soleus, SOL) and fast-twitch (biceps femoris, BF) muscle to identify differences between these muscle types.

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ALCAR supplementation to unloaded soleus muscle

Project description:The effect of acetyl-L-carnitine (ALCAR) supplementation to hindlimb suspended rats has been analyzed in order to test whether this would promote the differentiation towards an oxidative phenotype of muscle fibers in soleus muscle and prevent the transformation of this slow tonic muscle towards a glycolytic, low-oxidative phenotype due to unloading condition. 3 month-old rats were daily supplemented with ALCAR concomitantly with hindlimb suspension. Validated microarray analysis for a broad range of muscle-relevant processes delineated an ALCAR-induced adjustment of the expression profile. In particular, transcript elevations of factors underlying oxidative metabolism in mitochondria and protein turnover by ribosomal and proteasomal components as well as an adaptation of voltage-gated ion channel expression were identified as an ALCAR response in soleus muscle. Keywords: ALCAR effect

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Transcription profiling of rat young soleus muscle (3-4 months) vssoleus muscle (30-31 months)

Project description:Comparison of gene expression in young soleus muscle (3-4 months) with old soleus muscle (30-31 months).

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Effect of acute muscle unloading on soleus and gastrocnemius muscle gene expression

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