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Effect of depletion of OASIS on gene expression in primary cultured mouse astrocytes during DNA damage response.

ABSTRACT: Effect of depletion of OASIS on gene expression in primary cultured mouse astrocytes during DNA damage response.

PROVIDER: PRJNA954723 | ENA |

REPOSITORIES: ENA

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Effect of depletion of OASIS on gene expression in primary cultured mouse astrocytes during DNA damage response.

Project description:To investigate the roles of OASIS/CREB3L1 in astrocytes during DNA damage response, we established OASIS wild type and deficient primary cultured mouse astrocytes treated with or without anti-cancer drug doxorubicin. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells.

2023-04-17 | GSE229480 | GEO

OASIS overexpression in murine podocyte cell line

Project description:Podocyte injury is involved in the onset and progression of various kidney diseases. We previously demonstrated that the transcription factor, old astrocyte specifically induced substance (OASIS) in myofibroblasts, contributes to kidney fibrosis, as a novel role of OASIS in the kidneys. Importantly, we found that OASIS is also expressed in podocytes; however, the pathophysiological significance of OASIS in podocytes remains unknown. Upon lipopolysaccharide (LPS) treatment, there is an increase in OASIS in murine podocytes. Enhanced serum creatinine levels and tubular injury, but not albuminuria and podocyte injury, are attenuated upon podocyte-restricted OASIS knockout in LPS-treated mice, as well as diabetic mice. The protective effects of podocyte-specific OASIS deficiency on tubular injury are mediated by protein kinase C iota (PRKCI/PKCι), which is negatively regulated by OASIS in podocytes. Furthermore, podocyte-restricted OASIS transgenic mice show tubular injury and tubulointerstitial fibrosis, with severe albuminuria and podocyte degeneration. Finally, there is an increase in OASIS-positive podocytes in the glomeruli of patients with minimal change nephrotic syndrome and diabetic nephropathy. Taken together, OASIS in podocytes contributes to podocyte and/or tubular injury, in part through decreased PRKCI. The induction of OASIS in podocytes is a critical event for the disturbance of kidney homeostasis.

2022-06-22 | GSE206525 | GEO

Microarray of RNA from calvaria in WT and OASIS-/- (KO) mice

Project description:Investigation of whole genome gene expression level changes in OASIS KO calvaria compared to wild-type calvaria. To gain further insight into the potential mechanisms underlying the defective bone formation in OASIS KO mice, we compared the gene expression in calvaria between WT and OASIS KO mice using a microarray. Each sample of total RNA was collected from a number of mice.

2010-05-05 | E-GEOD-18062 | biostudies-arrayexpress

Microarray of RNA from calvaria in WT and OASIS-/- (KO) mice

2009-09-10 | GSE18062 | GEO

oasis metagenome

Project description:oasis metagenome Metagenome

| PRJNA321859 | ENA

Rat Astrocyte-Neuron Co-cultures versus purified co-cultured Astrocytes versus Astrocyte alone

Project description:Profiling of transcriptional changes in rat astrocytes when co-cultured with neurons: comparison of astrocytes cultured alone with astrocytes co-cultured with mouse hippocampal neurons. Co-cultured astrocytes are isolated using cold jet, a novel tool for these neuron-glia cultures. Over the last decade, the importance of astrocyte-neuron communication in neuronal development and synaptic plasticity has become increasingly clear. Since neuron-astrocyte interactions represent highly dynamic and reciprocal processes, we hypothesized that at least part of the involved astrocyte genes may be regulated as a consequence of their interactions with maturing neurons. In order to identify such neuron-induced astrocyte genes in vitro, we tested the effectiveness of the ‘cold jet’, a new method for separation of neurons from co-cultured astrocytes. The cold jet method is performed under ice-cold conditions and avoids protease-mediated isolation of astrocytes or time-consuming centrifugation, yielding intact astrocyte mRNA with approximately 90% of neuronal RNA removed. Using this method, we executed genome-wide profiling in which RNA derived from astrocyte-only cultures was compared with astrocyte RNA derived from differentiating neuron-astrocyte co-cultures. Data analysis revealed changes in expression of a large number of mRNAs and biological processes, including novel findings. Thus, cold jet is an efficient method to separate astrocytes from neurons in co-culture, and in this study reveals that neurons induce robust gene-expression changes in co-cultured astrocytes.

2016-11-09 | GSE52481 | GEO

Transcriptional profile (RNA-seq) of cultured astrocytes [RegionAstro]

Project description:Transcriptional profile (RNA-seq) of cultured astrocytes [RegionAstro]

| PRJNA727799 | ENA

oasis metagenome

Project description:Desert Oasis strain:community Targeted loci environmental

| PRJNA311559 | ENA

Gene expression of human cancer cells co-cultured with mouse astrocytes and fibroblasts after series of cross co-culture

Project description:Brain metastases are highly resistant to chemotherapy. Brain metastases are surrounded and infiltrated by activated astrocytes. To examine the genes whose expression is associated with chemo-resistance of brain-metastasized cancer cells, gene expression data were collected and analyzed from breast cancer cells and lung cancer cells co-cultured with astrocytes. Fibroblast cells were used as control. Human lung cancer cell PC14 was co-cultured with mouse astrocytes or fibroblasts for two rounds. Total RNAs were extracted from co-cultured cells and hybridized to human microarray.

2012-12-31 | E-GEOD-26292 | biostudies-arrayexpress

Differential expression of genes cultured on extracellular matrix from control or irradiated astrocytes

Project description:U3082 human glioma cells were cultured on cell-derived extracellular matrix from primary human astrocytes that had been treated with either 0 Gy or 10 Gy radiation.

2021-01-25 | GSE145060 | GEO

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