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ChIP-reChIP-seq data in mESCs show ZFP661 co-binds target regions with CTCF

ABSTRACT: ChIP-reChIP-seq data in mESCs show ZFP661 co-binds target regions with CTCF

PROVIDER: PRJNA968172 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24461848_1.fastq.gz	Fastqsanger.gz
	SRR24461848_2.fastq.gz	Fastqsanger.gz
	SRR24461849_1.fastq.gz	Fastqsanger.gz
	SRR24461849_2.fastq.gz	Fastqsanger.gz
	SRR24461850_1.fastq.gz	Fastqsanger.gz

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ChIP-reChIP-seq data in mESCs show ZFP661 co-binds target regions with CTCF

Project description:In this assay, we aimed to investigate whether ZFP661 co-binds target regions with CTCF. To achieve this, we performed ChIP-reChIP-seq in ZFP661-3HA overexpressing (OE) mESCs, where the 1st ChIP utilized anti-HA antibody, and the 2nd ChIP involved the use of anti-IgG and anti-CTCF antibodies, respectively. Our results demonstrate that ZFP661 co-binds target regions with CTCF.

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Project description:In this assay, we aimed to investigate the occupancy of ZFP661 in the mouse genome and its possible role in regulating the occupancies of CTCF and cohesin, which are two key factors in establishing 3D chromatin structures. To achieve this, we performed ChIP-seq in ZFP661-3HA endogenously tagged mESCs (for ZFP661), Zfp661 knockout (KO) mESCs (for CTCF and RAD21), and ZFP661-3HA overexpressing (OE) mESCs (for ZFP661, CTCF, RAD21, KAP1, and H3K9me3). Our results reveal that 1) ZFP661 binding peaks were co-occupied with those of CTCF; 2) ZFP661 binds exclusively inside the CTCF loop anchors; and 3) ZFP661 can suppress cohesin binding at CTCF barriers without altering CTCF binding.

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Project description:ChIP-seq data in mESCs show ZFP661 suppresses cohesin binding at CTCF barriers

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