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CITE-seq of LSK cells from aged mice after long-term iron chelator regimen

ABSTRACT: CITE-seq of LSK cells from aged mice after long-term iron chelator regimen

PROVIDER: PRJNA970509 | ENA |

REPOSITORIES: ENA

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CITE-seq of LSK cells from aged mice after long-term iron chelator regimen

Project description:We found that iron chelation restored functional defects in aged HSC, including engraftment potential and platelet bias. To gain molecular insights into iron-dependent mechanism for sustaining HSC identity during aging, we performed Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) with lineage (Lin)− Sca-1+ cKit+ (LSK) cells isolated from aged mice after long-term regimens with iron chelator Deferoxamine or vehicle control.

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CITE-seq analysis of mouse LSK cells

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CITE-seq analysis of mouse LSK cells

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Project description:Using bulk RNA-seq, the transcriptional profile of two cell lines with high or low metastatic properties derived from single clones of the murine 4T1 cell line treated or untreated with iron chelator DFX was analyzed in order to determine the mechanism underlying the inhibition preference of DFX for highly metastatic 4T1 cells.

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Transcription profiling of human HeLa cells grown to subconfluent density and CaCo-2 cells grown to high density treated with an iron source (hemin) or an iron chelator (desferal)

Project description:HeLa cells grown to subconfluent density and CaCo-2 cells grown to high density were either treated with an iron source (hemin) or an iron chelator (desferal). Cells were harvested and RNA was prepared by RNA-Clean (Hybaid-AGS) and the RNA was subjected to a subsequent clean up using the RNeasy (Qiagen) clean up procedure.

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Taz protects aged hematopoietic stem cells from exhaustion [CITE-seq]

2022-07-14 | GSE157458 | GEO

Treatment of a human lymphoblastoid cell line with the iron chelator deferoxamine

Project description:Treatment of a human lymphoblastoid cell line with the iron chelator deferoxamine

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Taz protects aged hematopoietic stem cells from exhaustion [bulk RNA-seq]

Project description:We report here the analysis of young and aged hematopoietic stem and progenitor cells (HSPCs) by transcriptional profiling (bulk RNA-Seq, CITE-Seq) and chromatin profiling (ATAC-Seq, scATAC-Seq). We use here a Clca3a1 as a surface marker to further fractionate HSPCs. HSPCs were sorted by flow cytometry. Where indicated, LT-HSCs were further fractionated into a Clca3a1 high or low population using a monoclonal antibody (10.1.1) purchased from the Developmental Studies Hybridoma Bank - DSHB. We report here the analysis of old LSK cells after Taz S89A overexpression. LSK cells were sorted by flow cytometry. The cells were infected in vitro with the indicated constructs and subjected to RNA-Seq analysis. We report here the analysis of BM-HPC#5 cells after PU1 knockdown. BM-HPC#5 cells with the indicated constructs were subjected to RNA-Seq analysis.

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