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A common precursor expression program leading to brain pericyte differentiation from neural crest and mesoderm

ABSTRACT: A common precursor expression program leading to brain pericyte differentiation from neural crest and mesoderm

PROVIDER: PRJNA973709 | ENA |

REPOSITORIES: ENA

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scRNAseq datasets supporting a common precursor expression program leading to brain pericyte differentiation from neural crest and mesoderm

Project description:Brain pericytes are critical for regulating endothelial barrier function and activity, thus ensuring adequate blood flow to the brain. How the developmental acquisition of pericytes to naked endothelium is regulated, is largely unknown, but is relevant to disorders where vessels are poorly stabilized. Although pericytes are derived from neural crest and mesoderm, brain pericytes from both origins are currently indistinguishable; the genetic pathways leading to a convergent pericyte phenotype are unknown. We show here that a precursor population expressing the transcription factor nkx3.1 with origins in both NCC and mesoderm, develops into brain pericytes. We identify the gene signature of these precursors and show that an nkx3.1, foxf2a, and cxcl12b -expressing pericyte precursor population is present around the cxcr4 expressing basilar artery, and that these cells later spread throughout the brain. Cxcl12b- Cxcr4 signaling is required for pericyte attachment and differentiation but not for later pericyte development. Further, both nkx3.1 and cxcl12b are necessary and sufficient in regulating pericyte number. Thus, we have defined an undescribed population of pericyte precursors and identified genes critical for their differentiation.

2024-02-25 | GSE232163 | GEO

A common precursor expression program leading to brain pericyte differentiation from neural crest and mesoderm

Project description:Brain pericytes are one of the critical cell types that regulate endothelial barrier function and activity, thus ensuring adequate blood flow to the brain. The genetic pathways guiding undifferentiated cells into mature pericytes are not well understood. We show here that pericyte precursor populations from both neural crest and head mesoderm of zebrafish express the transcription factor nkx3.1 develop into brain pericytes. We identify the gene signature of these precursors and show that an nkx3.1-, foxf2a-, and cxcl12b-expressing pericyte precursor population is present around the basilar artery prior to artery formation and pericyte recruitment. The precursors later spread throughout the brain and differentiate to express canonical pericyte markers. Cxcl12b-Cxcr4 signaling is required for pericyte attachment and differentiation. Further, both nkx3.1 and cxcl12b are necessary and sufficient in regulating pericyte number as loss inhibits and gain increases pericyte number. Through genetic experiments, we have defined a precursor population for brain pericytes and identified genes critical for their differentiation. This SuperSeries is composed of the SubSeries listed below.

2024-02-25 | GSE232763 | GEO

Bulk RNA-Seq datasets supporting a common precursor expression program leading to brain pericyte differentiation from neural crest and mesoderm

2024-02-25 | GSE232762 | GEO

scRNAseq datasets supporting a common precursor expression program leading to brain pericyte differentiation from neural crest and mesoderm

Project description:scRNAseq datasets supporting a common precursor expression program leading to brain pericyte differentiation from neural crest and mesoderm

| PRJNA971152 | ENA

Bulk RNA-Seq datasets supporting a common precursor expression program leading to brain pericyte differentiation from neural crest and mesoderm

Project description:Bulk RNA-Seq datasets supporting a common precursor expression program leading to brain pericyte differentiation from neural crest and mesoderm

| PRJNA973714 | ENA

A mesoderm-derived mesenchymal stem/stromal cells (MSC) precursor

Project description:This SuperSeries is composed of the following subset Series: GSE20045: A mesoderm-derived mesenchymal stem/stromal cells (MSC) precursor: time course experiment GSE20046: A mesoderm-derived mesenchymal stem/stromal cells (MSC) precursor: stages of development experiment Refer to individual Series

2010-12-03 | E-GEOD-20047 | biostudies-arrayexpress

Single cell gene expression of early E8.5 pioneer neural crest cells and paraxial mesoderm

Project description:Laser capture microdissection (LCM) was used to isolate cells from the principal critical micro-regions, whose development, differentiation and signaling interactions are responsible for the construction of the mammalian face. At E8.5, as migrating neural crest cells begin to exit the neural fold/epidermal ectoderm boundary, we examined the facial mesenchyme, composed of neural crest and paraxial mesoderm cells, as well as cells from adjacent neuroepithelium We performed single cell studies to better define the gene expression states of the early E8.5 pioneer neural crest cells and paraxial mesoderm, and present microarray data detailing expression patterns within these embryonic cell populations. Mouse emrbyos were harvested at developmental stage E8.5 and single cells were captured from the neuroepithilium, neural crest, and paraxial mesoderm. RNA was extracted, labelled, and quantified using the Mouse ST-l microarray.

2014-03-18 | E-GEOD-55964 | biostudies-arrayexpress

RNA-seq analysis of hPSC-derived brain pericyte-like cells, hPSC-derived neural crest stem cells, and primary human brain pericytes

Project description:hPSCs were differentiated to brain pericyte-like cells through a neural crest stem cell intermediate and compared to primary human brain pericytes

2019-03-13 | GSE124579 | GEO

Single cell gene expression of early E8.5 pioneer neural crest cells and paraxial mesoderm

Project description:Laser capture microdissection (LCM) was used to isolate cells from the principal critical micro-regions, whose development, differentiation and signaling interactions are responsible for the construction of the mammalian face. At E8.5, as migrating neural crest cells begin to exit the neural fold/epidermal ectoderm boundary, we examined the facial mesenchyme, composed of neural crest and paraxial mesoderm cells, as well as cells from adjacent neuroepithelium. For these 11 samples, we used a single cell isolation and cDNA generation protocol followed by a standard Affymetrix labeling protocol to better define the gene expression states of the early E8.5 pioneer neural crest and paraxial mesoderm cells. Using a combination of statistical and expression level filtering criteria, we have identified expression patterns present within these embryonic cell populations. The genes identified through this study are highly enriched for known critical genes as well as many genes not previously known to be cell type specific at this stage.

2014-03-18 | GSE55964 | GEO

Expression profiling of the murine neural crest precursor cell line, JoMa1

Project description:JoMa1 cells are pluripotent precursor cells, derived from the neural crest of mice transgenic for tamoxifen-inducible c-Myc. Following transfection with a cDNA encoding for MYCN, cells become immortlized even in the absence of tamoxifen. The expression profile of JoMa1 cells was compared to cell lines derived from tumors induced by JoMa1 transfected with MYCN.

2012-04-29 | E-GEOD-27159 | biostudies-arrayexpress

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