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An atypical GdpP enzyme linking cyclic nucleotide metabolism to gene regulation in Mycoplasma bovis

ABSTRACT: An atypical GdpP enzyme linking cyclic nucleotide metabolism to gene regulation in Mycoplasma bovis

PROVIDER: PRJNA975114 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24685466_1.fastq.gz	Fastqsanger.gz
	SRR24685466_2.fastq.gz	Fastqsanger.gz
	SRR24685467_1.fastq.gz	Fastqsanger.gz
	SRR24685467_2.fastq.gz	Fastqsanger.gz
	SRR24685468_1.fastq.gz	Fastqsanger.gz

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An atypical GdpP enzyme linking cyclic nucleotide metabolism to gene regulation in Mycoplasma bovis

Project description:To better understand the role of MbovGdpP in cellular processes, the expression profiles of differentially expressed genes between M. bovis wild type strain HB0801 and MbovGdpP knock-out mutant were determined by RNA-sequencing (RNA-seq). Differential gene expression analysis identified up to 161 genes with significant changes in T6.290 (|log2 fold change| > 1.5 and p < 0.05), with 74 mRNA up-regulated and 87 down-regulated. Transcriptomic analyses revealed the biological importance of MbovGdpP.

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An atypical GdpP enzyme linking cyclic nucleotide metabolism to osmotic tolerance and gene regulation in <i>Mycoplasma bovis</i>.

Project description:Nucleotide second messengers play an important role in bacterial adaptation to environmental changes. Recent evidence suggests that some of these regulatory molecular pathways were conserved upon the degenerative evolution of the wall-less mycoplasmas. We have recently reported the occurrence of a phosphodiesterase (PDE) in the ruminant pathogen Mycoplasma bovis, which was involved in c-di-AMP metabolism. In the present study, we demonstrate that the genome of this mycoplasma species encodes a PDE of the GdpP family with atypical DHH domains. Characterization of M. bovis GdpP (MbovGdpP) revealed a multifunctional PDE with unusual nanoRNase and single-stranded DNase activities. The alarmone ppGpp was found unable to inhibit c-di-NMP degradation by MbovGdpP but efficiently blocked its nanoRNase activity. Remarkably, MbovGdpP was found critical for the osmotic tolerance of M. bovis under K+ and Na+ conditions. Transcriptomic analyses further revealed the biological importance of MbovGdpP in tRNA biosynthesis, pyruvate metabolism, and several steps in genetic information processing. This study is an important step in understanding the role of PDE and nucleotide second messengers in the biology of a minimal bacterial pathogen.

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