Project description:To investigate the function of NleE in the regulation of host transcription, we established HCT116 cell lines stably expressing NleE. We performed toal RNA-seq and compared transcription of HCT116 cells with and without NleE expression.
Project description:We characterized the marine natural product cortistatin A (CA) as an inhibitor of CDK8 to determine whether pharmacologic inhibition of CDK8 regulates super-enhancer function and inhibits AML proliferation. In this series, we examine the transcriptional effect on insensitive HCT116 cells of 3hrs exposure to CA.