Ontology highlight
ABSTRACT:
PROVIDER: PRJNA978348 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR24788100_1.fastq.gz | Fastqsanger.gz | |||
| SRR24788100_2.fastq.gz | Fastqsanger.gz | |||
| SRR24788101_1.fastq.gz | Fastqsanger.gz | |||
| SRR24788101_2.fastq.gz | Fastqsanger.gz | |||
| SRR24788102_1.fastq.gz | Fastqsanger.gz |
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Project description:effect of biocide adaptation on antimicrobial resistance in Listeria monocytogenese
| PRJEB48925 | ENA
Project description:Biocides are chemical compounds widely used in hospital settings for a variety of purposes, but mainly for disinfection. The chemical properties of a biocide, as well as the biocide concentration, influence which cellular targets are affected. Exposure of bacteria to residual concentrations of biocides could lead to development of increased resistance towards the biocide in use, as well as cross-resistance towards other antimicrobials, including antibiotics. The aim of this study was to examine whether biocides could induce any potentially relevant genes that could affect pathogen's drug resistance or fitness. By examining global gene expression of the uropathogenic Escherichia coli CFT073 after exposure to subinhibitory concentrations of five biocides (benzalkonium chloride - BAC, chlorhexidine - CHX, ethanol - EtOH, hydrogen peroxide - H2O2, triclosan - TSN), we found that each biocide changed expression of different groups of genes and that exposure to ethanol caused changes in expression of the largest number of genes among all biocides. In general, the five biocides tested in this study at subinhibitory concentrations did not increase the resistance potential of the pathogen to other antimicrobials. We could, however, identify clusters of genes that could possibly help the strain to grow in the presence of a biocide in the medium. A culture of E. coli CFT073 without any biocide treatment served as the control sample. That culture was grown under the exact same conditions as the five biocide-treated samples. Each sample was collected in three biological replicates at the mid-exponential phase of growth.
2016-03-01 | E-GEOD-72738 | biostudies-arrayexpress
Project description:Biocides are chemical compounds widely used in hospital settings for a variety of purposes, but mainly for disinfection. The chemical properties of a biocide, as well as the biocide concentration, influence which cellular targets are affected. Exposure of bacteria to residual concentrations of biocides could lead to development of increased resistance towards the biocide in use, as well as cross-resistance towards other antimicrobials, including antibiotics. The aim of this study was to examine whether biocides could induce any potentially relevant genes that could affect pathogen's drug resistance or fitness. By examining global gene expression of the uropathogenic Escherichia coli CFT073 after exposure to subinhibitory concentrations of four biocides (benzalkonium chloride - BAC, chlorhexidine - CHX, hydrogen peroxide - H2O2, triclosan - TSN), we found that each biocide changed expression of different groups of genes and that exposure to benzalkonum chloride caused changes in expression of the largest number of genes among all biocides. In general, the four biocides tested in this study at subinhibitory concentrations did not increase the resistance potential of the pathogen to other antimicrobials. We could, however, identify clusters of genes that could possibly help the strain to grow in the presence of a biocide in the medium.
2019-12-05 | GSE135556 | GEO
Project description:The objective of the current study was to understand the glutaraldehyde resistance mechanisms in P. fluorescens and P. aeruginosa biofilms. Glutaraldehyde is a common biocide used in various industries to control the microbial growth. Recent reports of emergence of glutaraldehyde resistance in several bacterial species motivated this study to understand the genetic factors responsible got glutaraldehyde resistance. Using a combination of phenotypic assays, chemical genetic assays and RNA-seq, we demonstrate that novel efflux pump, polyamine biosynthesis, lipid biosynthesis and phosphonate degradation play significant role in glutaraldehyde resistance and post-glutaraldehyde recovery of Psudomonad biofilms.
2015-04-06 | GSE64448 | GEO
Project description:The impacts of man-made chemicals, in particular of persistent organic pollutants, are multifactorial as they may affect the integrity of ecosystems, alter biodiversity and hinder the health of most organisms. We have demonstrated that the belowground mycobiota of forest soils acts as a buffer against the pollution provoked by the biocide pentachlorophenol. However the trade-offs of the mitigation of the pollutant remain cryptic. To address this question we scrutinised the key changing aspects of a metacommunity of fungi when confronted (or not) with the biocide, comprising taxonomic and functional levels, as well as the identification of the major pollutant degraders within the metacommunity. Exposure to the biocide led to alterations in the metacommunity composition and functioning, many of which were not fully alleviated when most of the biocide was degraded, especially the dysregulation of the carbon and nitrogen metabolisms. The last is possibly linked to the higher pathogenic potential of the metacommunity after exposure to the biocide, supported by the secretion of proteins related to pathogenicity and reduced susceptibility to a standard fungicide. Our findings provide additional evidence for the silent risks of environmental pollution, particularly as it may favour the development of pathogenic trade-offs in fungi, which may impose serious threats to animals and plant hosts.
2018-11-28 | PXD009798 | Pride
Project description:The objective of the current study was to understand the glutaraldehyde resistance mechanisms in P. fluorescens and P. aeruginosa biofilms. Glutaraldehyde is a common biocide used in various industries to control the microbial growth. Recent reports of emergence of glutaraldehyde resistance in several bacterial species motivated this study to understand the genetic factors responsible got glutaraldehyde resistance. Using a combination of phenotypic assays, chemical genetic assays and RNA-seq, we demonstrate that novel efflux pump, polyamine biosynthesis, lipid biosynthesis and phosphonate degradation play significant role in glutaraldehyde resistance and post-glutaraldehyde recovery of Psudomonad biofilms. Examination of P. fluorescens 72 h biofilm transcriptome was elucidated upon exposure to glutaraldehyde. The results were confirmed using qRT--PCR and chemical genetic appraoches in P. fluorescens and P. aeruginosa.
2015-04-06 | E-GEOD-64448 | biostudies-arrayexpress
Project description:Listeria monocytogenes is a foodborne intracellular bacterial pathogen leading to human listeriosis. Despite a high mortality rate and increasing antibiotic resistance no clinically approved vaccine against Listeria is available. To identify antigens for this bacterial pathogen that can be encoded in mRNA vaccine formulations, we screened for Listeria epitopes presented on the surface of infected human cell lines by mass spectrometry-based immunopeptidomics. In between more than 15,000 human self-peptides, we detected 68 Listeria epitopes from 42 different bacterial proteins, including several known antigens. Peptide epitopes presented on different cell lines were often derived from the same bacterial surface proteins, classifying these antigens as potential vaccine candidates. Encoding these highly presented antigens in lipid nanoparticle mRNA vaccine formulations resulted in high levels of protection in vaccination challenge experiments in mice. Our results pave the way for the development of a clinical mRNA vaccine against Listeria and demonstrate the power of immunopeptidomics for next-generation bacterial vaccine development.
2022-09-26 | PXD031451 | Pride
Project description:The global response of E. coli tot he broad spectrum biocide polyhexamethyelene biguanide (PHMB) was investigated using transcriptional profiling. Keywords: dose response
2006-02-01 | GSE2827 | GEO
Project description:CadC is the transcriptional regulator of CadA, an efflux pump conferring cadmium resistance. We previously showed that during in vivo infection, Listeria monocytogenes uses CadC to directly repress the expression of the LspB lipoprotein signal peptidase, avoiding the exposure of the lipoprotein LpeA to the host immune system, impairing inflammatory cytokine expression and promoting intramacrophage survival and virulence. Here, to assess if CadC could be a widespread virulence regulator, we searched for other genes regulated by CadC using Listeria monocytogenes tiling arrays.
2022-04-04 | E-MTAB-7857 | biostudies-arrayexpress