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Whole-transcriptome RNA sequencing and degradome sequencing were performed on maize inbred lines Chang7-2 (resistant to SCMV) and Mo17 (susceptible to SCMV).

ABSTRACT: Whole-transcriptome RNA sequencing and degradome sequencing were performed on maize inbred lines Chang7-2 (resistant to SCMV) and Mo17 (susceptible to SCMV).

PROVIDER: PRJNA978633 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24800038_1.fastq.gz	Fastqsanger.gz
	SRR24800038_2.fastq.gz	Fastqsanger.gz
	SRR24800039_1.fastq.gz	Fastqsanger.gz
	SRR24800039_2.fastq.gz	Fastqsanger.gz
	SRR24800040_1.fastq.gz	Fastqsanger.gz

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Degradome sequencing were performed on maize inbred lines Chang7-2 (resistant to SCMV) and Mo17 (susceptible to SCMV).

Project description:The maize inbred lines Chang7-2 (resistant to SCMV) and Mo17 (susceptible to SCMV) were inoculated with SCMV (SC, SM) and phosphate buffer (MC, MM), respectively to subjected to degradome sequencing.

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Whole-transcriptome RNA sequencing and degradome sequencing were performed on maize inbred lines Chang7-2 (resistant to SCMV) and Mo17 (susceptible to SCMV).

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Degradome sequencing were performed on maize inbred lines Chang7-2 (resistant to SCMV) and Mo17 (susceptible to SCMV).

Project description:Degradome sequencing were performed on maize inbred lines Chang7-2 (resistant to SCMV) and Mo17 (susceptible to SCMV).

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Transcription profiling of maize inbred lines B73 and Mo17

Project description:Microarray analysis of gene expression patterns in immature ear, seedling, and embryo tissues from the maize inbred lines B73 and Mo17 identified numerous genes with variable expression. Some genes had detectable expression in only one of the two inbreds; most of these genes were detected in the genomic DNA of both inbreds, indicating that the expression differences are likely caused by differential regulation rather than by differences in gene content. Gene expression was also monitored in the reciprocal F1 hybrids B73xMo17 and Mo17xB73. The reciprocal F1 hybrid lines did not display parental effects on gene expression levels. Approximately 80% of the differentially expressed genes displayed additive expression patterns in the hybrids relative to the inbred parents. The approximately 20% of genes that display nonadditive expression patterns tend to be expressed at levels within the parental range, with minimal evidence for novel expression levels greater than the high parent or less than the low parent. Analysis of allele-specific expression patterns in the hybrid suggested that intraspecific variation in gene expression levels is largely attributable to cis-regulatory variation in maize. Collectively, our data suggest that allelic cis-regulatory variation between B73 and Mo17 dictates maintenance of inbred allelic expression levels in the F1 hybrid, resulting in additive expression patterns. Experiment Overall Design: Affymetrix expression profiling was used to study gene expression in immature ear tissue from maize. Three biological replicates were performed for four different genotypes; B73, Mo17, B73xMo17 and Mo17xB73.

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Project description:We aimed to identify targets of miRNAs during wheat grain development by using degradome sequencing approach. Two degradome libraries were constructed from wheat grains. Verification of miRNA targets from two degradome libraries in developing wheat grains.

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Maize single base resolution methylome in B73 and Mo17 inbred line and their reciprocal hybrid

Project description:Here we report genome-wide high resolution allele-specific maps of DNA methylation and histone H3 lysine 27 trimethylation (H3K27me3) in maize endosperm. To investigate the allele-specific DNA methylation pattern of maize endosperm on a genome-wide scale, we performed MethylC-seq for shoot, embryo, and endosperm tissue 12 d after pollination (DAP) of inbred B73, and the endosperm tissue 12 DAP of reciprocal crosses B73 Ã Mo17 (BM) and Mo17 Ã B73 (MB). We also performed additional RNA-seq for samples from 12-DAP and 10-DAP endosperm of both reciprocal crosses between inbreds B73 and Mo17

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Sequencing of rice degradome

Project description:miRNAs can regulate target gene expression by mRNA cleavage. Rice degradome sequencing was employed to validate mRNA targets of rice miRNAs. Two rice samples, 3-week-old seedling and young panicles were included in the study.

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Project description:We aimed to identify targets of miRNAs during wheat grain development by using degradome sequencing approach. Two degradome libraries were constructed from wheat grains.

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Project description:miRNAs can regulate target gene expression by mRNA cleavage. Rice degradome sequencing was employed to validate mRNA targets of rice miRNAs.

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Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression - Embryo data

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