Project description:This Series involves two studies: 1) The gene expression of E. coli K-12 BW25113 ompA mutant strain vs. wild type strain glasswool biofilm cells and E. coli K-12 BW25113 ompA mutant vs. wild type polystyrene biofilm cells. 2) The gene expression of E. coli BW25113 ompA/pCA24N_ompA vs. ompA/pCA24N suspension cells.
Project description:E. coli K-12 wild type strains gene expression in biofilm cells with R1drd19 relative to that in biofilm cells of E. coli K-12 wild type strains without R1drd19. All the samples were cultured in LB or M9C glu on glass wool at different time. Keywords: biofilm
Project description:This Series involves two studies: 1) The gene expression of E. coli K-12 BW25113 ompA mutant strain vs. wild type strain glasswool biofilm cells and E. coli K-12 BW25113 ompA mutant vs. wild type polystyrene biofilm cells. 2) The gene expression of E. coli BW25113 ompA/pCA24N_ompA vs. ompA/pCA24N suspension cells. Strains: E. coli K-12 BW25113 wild type, ompA mutant Medium: LB Cell type: Biofilm cells grown on glasswool and polystyrene surfaces Time: 15 h Temperature: 37C Strains: BW25113 ompA/pCA24N_ompA and ompA/pCA24N Medium: LB Time: 7 h Temperature: 37C Cell type: suspension cells, induced by 0.1 mM IPTG
Project description:Honey has been widely used against bacterial infection for centuries. Previous studies suggested that honeys in high concentrations inhibited bacterial growth due to the presence of anti-microbial compounds, such as methylglyoxal, hydrogen peroxide, and peptides. In this study, we found that three honeys (acacia, clover, and polyfloral) in a low concentration as below as 0.5% (v/v) significantly suppress virulence and biofilm formation in enterohemorrhagic E. coli O157:H7 affecting the growth of planktonic cells while these honeys do not harm commensal E. coli K-12 biofilm formation. Transcriptome analyses show that honeys (0.5%) markedly repress quorum sensing genes (e.g., AI-2 import and indole biosynthesis), virulence genes (e.g., LEE genes), and curli genes (csgBAC). We found that glucose and fructose in honeys are key compounds to reduce the biofilm formation of E. coli O157:H7 via suppressing curli production, but not that of E. coli K-12. Additionally, we observed the temperature-dependent response of honeys and glucose on commensal E. coli K-12 biofilm formation; honey and glucose increase E. coli K-12 biofilm formation at 37°C, while they decrease E. coli K-12 biofilm formation at 26°C. These results suggest that honey can be a practical tool for reducing virulence and colonization of the pathogenic E. coli O157:H7, while honeys do not harm commensal E. coli community in the human.
Project description:E. coli K-12 wild type strains gene expression in biofilm cells with R1drd19 relative to that in biofilm cells of E. coli K-12 wild type strains without R1drd19. All the samples were cultured in LB or M9C glu on glass wool at different time. Experiment Overall Design: Strains: E. coli K-12 strains with and without R1drd19 respectively; Experiment Overall Design: Medium: LB was used for BW25113 strains, and M9C glu was used for MG1655 strains; Experiment Overall Design: Biofilm grown on glass wool; Experiment Overall Design: Time: 7h, 15h, 24h respectively; Experiment Overall Design: Cell type: biofilm.
Project description:E. coli K-12 BW25113 mutant strain ycfR expression in biofilm cells relative to E. coli wild-type strain expression in biofilm cells. All samples cultured in LB glu with glass wool Keywords: Cell type comparison
Project description:E. coli K-12 ATCC 25404 in LB medium with 5-fluorouracil 10 uM biofilm cells relative to E. coli K-12 ATCC 25404 in LB DMF biofilm cells. The same amount of stock 5-fluoroacil stock solution (0.1% of the volume) was added as DMF into the LB DMF. Experiment Overall Design: Strain: E. coli K-12 ATCC 25404 wild-type Experiment Overall Design: Medium: LB Experiment Overall Design: Biofilm growth on glass wool Experiment Overall Design: Time: 24 h Experiment Overall Design: Cell type: biofilm Experiment Overall Design: 5-fluorouracil vs. DMF
